rifampin and Conjunctivitis--Inclusion

Topics: Animals; Anti-Bacterial Agents; Chick Embryo; Chlamydiaceae; Conjunctivitis, Inclusion; DNA Viruses; Fluorescent Antibody Technique; Hepatitis A; Hepatitis B Antigens; Humans; Immunologic Techniques; Keratitis, Dendritic; Meningitis, Viral; Methods; Microscopy, Electron; Rabbits; Recurrence; Rickettsia Infections; Rifampin; RNA Viruses; Sulfonamides; Trachoma; Virus Diseases

A double-blind stratified trial was carried out on 85 patients to assess the efficacy of topical therapy with 1% chlortetracycline eye ointment in comparison with 1% rifampicin eye ointment in the treatment of chlamydial ocular infection of sexually transmitted origin (paratrachoma). Patients included were selected on the basis of positive culture for Chlamydia trachomatis. A 6-week course of treatment with chlortetracycline or rifampicin 3 times daily gave a clinical cure rate of 80% and 75% and a microbiological cure rate of 93% and 86% respectively. In patients who were not cured the intensity of inflammatory responses was considerably reduced.

Topics: Administration, Topical; Adolescent; Adult; Chlamydia Infections; Chlamydia trachomatis; Chlortetracycline; Clinical Trials as Topic; Conjunctivitis, Inclusion; Double-Blind Method; Female; Humans; Keratoconjunctivitis; Male; Middle Aged; Random Allocation; Rifampin; Trachoma

In four patients with an adult chlamydial ophthalmia small, marginal corneal abscesses were detected. These corneal abscesses were associated with unilateral papillary and follicular conjunctivitis and punctate keratitis. In these patients no bacteria was isolated from the abscesses, but Chlamydia trachomatis was isolated from materials collected from the abscesses and from the conjunctival swabbings. In addition all patients had microbiologically proved concomitant chlamydial genital infections. The clinical signs resolved after topical treatment with rifampicin or tetracycline eye ointment for six weeks or systemic treatment with tetracycline for two weeks. Because of concomitant chlamydial genital infection it is advisable to treat patients with adult chlamydial ophthalmia with systemic tetracycline and to refer these patients and their consorts for investigation and treatment of their genital infection.

Topics: Abscess; Adult; Chlamydia Infections; Chlamydia trachomatis; Conjunctivitis, Inclusion; Corneal Diseases; Female; Humans; Keratitis; Male; Middle Aged; Rifampin; Tetracycline

Topics: Antibodies, Bacterial; Bacteriological Techniques; Centrifugation; Chlamydia; Chlamydia Infections; Chloramphenicol; Clinical Laboratory Techniques; Complement Fixation Tests; Conjunctiva; Conjunctivitis, Inclusion; Culture Media; Fluorescent Antibody Technique; Humans; Independent State of Samoa; Indonesia; Methods; Rifampin; Trachoma; Tunisia

Highly purified trachoma elementary bodies (T'ang strain), incubated in the presence of the four nucleoside triphosphates [Mg(2+), Mn(2+), 2-mercaptoethanol, tris(hydroxymethyl)aminomethane buffer (pH 7.5)] were found to incorporate (3)H-uridine triphosphate (UTP) into ribonucleic acid (RNA) molecules. Eighty-seven per cent of the labeled molecules were sensitive to ribonuclease treatment. In vitro RNA synthesis was almost completely inhibited by actinomycin D. Rifampin was also inhibitory, but allowed some initial RNA synthesis before complete inhibition occurred. When the reaction mixture lacked Mn(2+), trachoma elementary bodies synthesized, for a limited period, high-molecular-weight RNA species (23 to 24S, 16 to 17S, and 10 to 11S). Addition of 0.2 m NaCl to the same reaction mixture stimulated and prolonged (3)H-UTP incorporation into the same radioactive RNA species. Addition of 0.001 m Mn(2+) instead of NaCl also stimulated (3)H-UTP incorporation but prevented the synthesis of the high-molecular-weight RNA species.

Topics: Amnion; Buffers; Cell Line; Centrifugation, Density Gradient; Chlamydia; Conjunctivitis, Inclusion; Culture Media; Cytoplasm; Dactinomycin; Genetic Code; Genetics, Microbial; Humans; Magnesium; Manganese; Mercaptoethanol; Molecular Weight; Nucleotides; Ribonucleases; Rifampin; RNA Nucleotidyltransferases; RNA, Bacterial; Sodium Chloride; Sucrose; Trachoma; Tritium; Tromethamine; Uracil Nucleotides