rifampin and Cell-Transformation--Neoplastic

Topics: Adenoviridae; Animals; Antigens, Neoplasm; Antigens, Viral; Cell Membrane; Cell Transformation, Neoplastic; Child; DNA Viruses; DNA, Neoplasm; DNA, Viral; Herpesviridae; Humans; Mutation; Oncogenic Viruses; Papillomaviridae; Polyomaviridae; Polyomavirus; Reverse Transcriptase Inhibitors; Rifampin; RNA Viruses; RNA-Directed DNA Polymerase; RNA, Viral; Simian virus 40; Temperature

Topics: Carbon Isotopes; Cell Transformation, Neoplastic; Chromatin; Cytoplasm; DNA Replication; DNA, Bacterial; DNA, Viral; Electrophoresis; Histones; Oncogenic Viruses; Operon; Peptide Chain Initiation, Translational; Peptide Chain Termination, Translational; Rifampin; RNA, Bacterial; RNA, Messenger; RNA, Ribosomal; Transcription, Genetic; Transformation, Genetic

DNA-dependent RNA polymerase (EC 2.7.7.6) ACTIVITIES FROM NORMAL BHK-21/C13 cells and from BHK-21/C13 cells transformed by polyoma virus (PYY cells) were solubilized and fractionated on columns of DEAE-Sephadex. Various properties of the A and B enzymes from the two types of cell were compared. 1. The yields of polymerase relative to the DNA content of the nuclear preparations are similar for both cell types. 2. The ionic-strength optima of polymerases A and B are 12.5 mM and 100mM with respect to (NH4)2SO4 for both cell types. 3. The Mn2+/Mg2+ activity ratio (measured at the respective optimum for each cation) for polymerase A from BHK-21/C13 cells was 1.48 and for the polymerase A from PYY cells was 0.55. The corresponding ratios for polymerase B were 10.11 for BHK-21/C13 cells and 22.75 for PYY cells. 4. Minor differences in the ability of the A polymerases to transcribe native and denatured DNA templates were observed; such differences were not apparent when the B polymerases were compared. 5. All the polymerases were inhibited completely by actinomycin D and by rifampicin AF/013, but not markedly so by rifampicin. Alpha-amanitin inhibited polymerase B but not polymerase A.

Topics: Amanitins; Ammonium Sulfate; Animals; Cell Line; Cell Transformation, Neoplastic; Cricetinae; Dactinomycin; DNA; DNA-Directed RNA Polymerases; Kidney; Magnesium; Manganese; Polyomavirus; Rifampin; Templates, Genetic

The effect of 2',6'-dimethyl-N(4')-benzyl-N(4')- [desmethyl]rifampicin on viral transformation induced by two unrelated oncogenic viruses was compared. Transformation of Balb/3T3 cells by the small, DNA-containing papova virus simian virus 40 was completely normal under conditions where transformation by the large, RNA-containing oncornavirus murine sarcoma virus was inhibited more than 150-fold. For these experiments a resistant variant of Balb/3T3 was selected that grows well in high concentrations of the drug, is not dependent on the drug for growth, and is probably not blocked at the level of drug uptake. These data show that dimethyl-benzylrifampicin specifically inhibits oncornavirus-induced transformation rather than nonspecifically inhibiting cellular growth or transformation.

Topics: Animals; Benzyl Compounds; Cell Division; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Drug Resistance; Fibroblasts; Gammaretrovirus; Mice; Mice, Inbred BALB C; Oncogenic Viruses; Rifampin; Sarcoma; Simian virus 40

Topics: Animals; Antiviral Agents; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Chromatography, DEAE-Cellulose; DNA Nucleotidyltransferases; DNA-Directed RNA Polymerases; Gammaretrovirus; Humans; Mice; Oncogenic Viruses; Rifampin; Rifamycins; RNA Viruses; Sarcoma, Experimental; Spleen; Structure-Activity Relationship; Tritium

Topics: Amantadine; Animals; Antiviral Agents; Azauridine; Cell Transformation, Neoplastic; Cytarabine; Deoxyuridine; DNA-Directed RNA Polymerases; Herpesviridae Infections; Humans; Idoxuridine; Interferons; Protein Biosynthesis; Rifampin; Rifamycins; RNA-Directed DNA Polymerase; RNA, Viral; Streptovaricin; Structure-Activity Relationship; Thiosemicarbazones; Transcription, Genetic; Vaccinia virus

Topics: Animals; Asparaginase; Cell Transformation, Neoplastic; Dactinomycin; Leukemia Virus, Murine; Mice; Rauscher Virus; Rifampin; Spleen; Tissue Extracts

Topics: Animals; Cell Division; Cell Transformation, Neoplastic; Cells, Cultured; DNA Nucleotidyltransferases; Gammaretrovirus; Kidney; Moloney murine leukemia virus; Rats; Rauscher Virus; Reverse Transcriptase Inhibitors; Rifampin; Vesicular stomatitis Indiana virus

Topics: Animals; Antiviral Agents; Avian Leukosis Virus; Cats; Cell Differentiation; Cell Transformation, Neoplastic; Chemical Phenomena; Chemistry; DNA Nucleotidyltransferases; DNA Replication; DNA, Viral; Humans; Leukocytes; Lymphatic System; Neoplasms; Oncogenic Viruses; Retroviridae; Rifampin; RNA Viruses; RNA, Neoplasm; RNA, Viral; Time Factors; Viral Interference

Topics: Animals; Cell Line; Cell Transformation, Neoplastic; Leukemia Virus, Murine; Mice; Rifampin

Topics: Animals; Benzopyrenes; Binding Sites; Carcinogens; Cell Transformation, Neoplastic; Chemical Phenomena; Chemistry; Cytosine; DNA Nucleotidyltransferases; DNA, Viral; Enzyme Activation; Hydrocarbons; Magnetic Resonance Spectroscopy; Mice; Moloney murine leukemia virus; Neoplasms, Radiation-Induced; Oncogenic Viruses; Photic Stimulation; Rifampin; RNA Nucleotidyltransferases; RNA, Viral; Spectrum Analysis; Virus Replication

Topics: Animals; Avian Sarcoma Viruses; Cell Transformation, Neoplastic; Chick Embryo; Culture Techniques; Fibroblasts; Rifampin; Time Factors; Virus Replication

Rifampin partially inhibits focus formation and virus production in chick embryo fibroblasts infected with Bryan high-titer Rous sarcoma (RAV-1) virus. This inhibition occurs with exposure to rifampin during a critical period between day 1 and day 2 after infection. This suggests that the drug does not affect formation of the provirus or its transcription or translation after the "fixation" step, but it seems to affect one or more events which take place before fixation and activation of virus production.

Topics: Animals; Avian Sarcoma Viruses; Cell Transformation, Neoplastic; Chick Embryo; Culture Media; Culture Techniques; DNA; Fibroblasts; Genetic Code; Genetics, Microbial; Immune Sera; Rifampin; Time Factors; Virus Replication

Topics: Animals; Avian Sarcoma Viruses; Cell Transformation, Neoplastic; Cells, Cultured; Chick Embryo; Dealkylation; Depression, Chemical; Fibroblasts; Hexoses; Methylamines; Phenazines; Rifampin; Trypan Blue

Topics: Cell Transformation, Neoplastic; DNA Nucleotidyltransferases; DNA, Viral; Nucleic Acid Hybridization; Oncogenic Viruses; Polynucleotides; Rifampin; RNA Viruses; RNA, Viral; Species Specificity; Templates, Genetic; Viral Proteins; Virus Replication

Topics: Animals; Avian Sarcoma Viruses; Cell Transformation, Neoplastic; Chick Embryo; Culture Techniques; Fibroblasts; Rifampin; Tritium; Uridine; Virus Replication