ridostin and Hemorrhagic-Fever--Ebola

The effects of recombinant analog of natural Ebola virus protein vp24 in configurations virulent (vp24-ad) and avirulent (vp24-w) for guinea pigs on interferonogenesis were studied in vivo and in vitro. Amino acid differences were determined by His186 substitution in avirulent (nonlethal) configuration for Tyr in the virulent (lethal) one. Recombinant analogs vp24-w and vp24-ad inhibited interferonogenesis in vivo and in vitro. Inhibition by the two protein configurations was virtually the same.

Topics: Animals; Guinea Pigs; Hemorrhagic Fever, Ebola; Interferon Inducers; Interferons; Mice; Mice, Inbred BALB C; RNA, Double-Stranded; RNA, Fungal; Viral Proteins; Virus Replication

The study of the preventive and therapeutic action of some immunomodulators (ridostin, reaferon and polyribonate) used alone and in combinations was conducted on laboratory animals infected aerogenically by Marburg or Ebola virus. It was found that special preventive intranasal and intramuscular administration of ridostin provided protection of the animals infected by Marburg virus (p = 0.1) and an increase in their mean lifespan by 2.4 days (p = 0.15). In the Ebola infection combined administration of ridostin and reaferon caused an essential increase in the mean lifespan of the animals by 2.9 days (p = 0.04). None of the tested drugs had any significant positive effect when used in various combinations according to the treatment schemes in Marburg and Ebola infections in guinea pigs.

Topics: Adjuvants, Immunologic; Aerosols; Animals; Drug Evaluation, Preclinical; Drug Therapy, Combination; Guinea Pigs; Hemorrhagic Fever, Ebola; Interferon alpha-2; Interferon Type I; Interferon-alpha; Marburg Virus Disease; Organic Chemicals; Recombinant Proteins; RNA, Double-Stranded; RNA, Fungal; Time Factors