rhyncophylline and Teratocarcinoma

rhyncophylline has been researched along with Teratocarcinoma* in 1 studies

Other Studies

1 other study(ies) available for rhyncophylline and Teratocarcinoma

Article	Year
Effect of rhynchophylline on apoptosis induced by dopamine in NT2 cells. Acta pharmacologica Sinica, 2002, Volume: 23, Issue:5 To study the effect of rhynchophylline (Rhy) on the apoptosis induced by dopamine (DA) in NT2 cell line.. Release of lactate dehydrogenase (LDH) from cultured cells was determined by enzyme-linked immunosorbant assay; apoptotic NT2 neurons by terminal de oxynucleotidyl transferase(TdT)-mediated dUTP nick end labeling method; DNA fragmentation by agarose gel electrophoresis; Bcl-2 protein expression by Western blotting.. DA 100 - 500 micromol/L increased release of LDH, number of apoptotic NT 2 neurons, and DNA fragmentation (P < 0.01). NT2 cells survival rate was decreased. Rhy 5 and 50 micromol/L reduced the percentage of apoptosis after treated with DA 100 micromol/L for 6 h in both with and without bcl-2 gene neurons (P < 0.05, P < 0.01, respectively). The expression of Bcl-2 protein was not affected by Rhy.. Rhy protected NT2 neurons from the damage induced by DA. Topics: Alkaloids; Apoptosis; Dopamine; Drug Interactions; Genes, bcl-2; Humans; Indole Alkaloids; Neurons; Neuroprotective Agents; Oxindoles; Teratocarcinoma; Transfection; Tumor Cells, Cultured	2002

Article

Year

Effect of rhynchophylline on apoptosis induced by dopamine in NT2 cells.

Acta pharmacologica Sinica, 2002, Volume: 23, Issue:5

To study the effect of rhynchophylline (Rhy) on the apoptosis induced by dopamine (DA) in NT2 cell line.. Release of lactate dehydrogenase (LDH) from cultured cells was determined by enzyme-linked immunosorbant assay; apoptotic NT2 neurons by terminal de oxynucleotidyl transferase(TdT)-mediated dUTP nick end labeling method; DNA fragmentation by agarose gel electrophoresis; Bcl-2 protein expression by Western blotting.. DA 100 - 500 micromol/L increased release of LDH, number of apoptotic NT 2 neurons, and DNA fragmentation (P < 0.01). NT2 cells survival rate was decreased. Rhy 5 and 50 micromol/L reduced the percentage of apoptosis after treated with DA 100 micromol/L for 6 h in both with and without bcl-2 gene neurons (P < 0.05, P < 0.01, respectively). The expression of Bcl-2 protein was not affected by Rhy.. Rhy protected NT2 neurons from the damage induced by DA.

Topics: Alkaloids; Apoptosis; Dopamine; Drug Interactions; Genes, bcl-2; Humans; Indole Alkaloids; Neurons; Neuroprotective Agents; Oxindoles; Teratocarcinoma; Transfection; Tumor Cells, Cultured

2002