rhodanine and Hepatitis--Chronic

The liver is the main storage site for copper. Excess copper accumulation, however, is a risk factor for the development of chronic hepatitis in dogs. Mass spectrometry or rhodanine staining are frequently used methods to assess copper levels in the liver. The association was studied between analytic hepatic copper levels and rhodanine scores in archived canine formalin-fixed-paraffinembedded liver sections from 2014 to 2021 with various diagnoses. Thirty-six (N = 36) liver samples with analytic interpretation of toxic (. Le foie est le principal site de stockage du cuivre. Cependant, une accumulation excessive de cuivre est un facteur de risque pour le développement d’une hépatite chronique chez le chien. La spectrométrie de masse ou la coloration à la rhodanine sont des méthodes fréquemment utilisées pour évaluer les niveaux de cuivre dans le foie. L’association entre les niveaux analytiques de cuivre hépatique et les scores de rhodanine a été étudiée dans des sections de foie de chien archivées fixées au formol et incluses dans de la paraffine de 2014 à 2021 avec divers diagnostics. Trente-six (N = 36) échantillons de foie avec interprétation analytique des niveaux de cuivre toxiques (

Topics: Animals; Copper; Dog Diseases; Dogs; Formaldehyde; Hepatitis, Chronic; Liver; Rhodanine

Based on 28 histologically well-established cases of chronic non-suppurative destructive cholangitis, clinico-pathological aspects of differential diagnosis and staging are reported. In only half of the cases could the diagnosis be made on the first liver biopsy. In 14 cases, two or more liver biopsy specimens were necessary for diagnosis. The most frequent and most difficult differential diagnostic issue was to distinguish between chronic non-suppurative destructive cholangitis and chronic aggressive hepatitis of viral or autoimmune origin. The histochemical demonstration of copper by the rhodanine method was of particular value for differentiating between chronic non-suppurative destructive cholangitis and chronic aggressive hepatitis as well as for diagnosis of mixed types. In two cases of mixed type, the HBs-Antigen in ground glass hepatocytes and copper-associated protein in periportal hepatocytes could be demonstrated simultaneously with Shikata's Orcein-staining. Compared with the previously used system (Scheuer 1967) the staging concept proposed by Ludwig et al. (1978) has proved to be more useful and easier to apply. This system permits recognition of the stage on routinely obtained specimens regardless of specific differential diagnostic features. Most liver biopsy specimens were assigned to stage III. Features considered characteristic of the earlier phases (inflammatory bile duct destruction and granulomas) frequently coexist with more advanced lesions in late stage.

Topics: Adolescent; Adult; Aged; Autoimmune Diseases; Biopsy; Copper; Diagnosis, Differential; Female; Hepatitis B Surface Antigens; Hepatitis, Chronic; Hepatitis, Viral, Human; Humans; Liver; Liver Cirrhosis, Biliary; Male; Middle Aged; Oxazines; Rhodanine