rhinacanthin-c and Cholangiocarcinoma

rhinacanthin-c has been researched along with Cholangiocarcinoma* in 1 studies

Other Studies

1 other study(ies) available for rhinacanthin-c and Cholangiocarcinoma

Article	Year
Rhinacanthin-C Extracted from Rhinacanthus nasutus (L.) Inhibits Cholangiocarcinoma Cell Migration and Invasion by Decreasing MMP-2, uPA, FAK and MAPK Pathways Asian Pacific journal of cancer prevention : APJCP, 2018, Dec-25, Volume: 19, Issue:12 Cholangiocarcinoma is a malignant tumor with high metastatic and mortality rates. We investigated the effects\ of rhinacanthin-C on cell proliferation, migration, invasion and the expression of proteins regulating cancer cell\ invasion-regulated proteins in a cholangiocarcinoma (KKU-M156) cell line. Cytotoxicity of rhinacanthin-C was\ determined by the SRB assay. Using wound-migration, chamber-migration and chamber-invasion assays, we assessed\ the effects of rhinacanthin-C against KKU-M156 cells. The activities of matrix metalloproteinases 2 and 9 (MMP-2,\ MMP-9) and urokinase-type plasminogen activator (uPA) were determined using gelatinase and uPA zymography\ assays. The expression of invasion-regulated proteins was investigated using western-blot analysis. After treatment\ with rhinacanthin-C, KKU-M156 cells exhibited antiproliferative effects in a dose-dependent manner with greater\ efficacy than in Vero cells: IC50 values were 1.50 and 2.37 μM, respectively. Rhinacanthin-C significantly inhibited cell\ migration and invasion of KKU-M156 cells in a dose-dependent manner. Consistent with this observation, treatment\ with rhinacanthin-C was associated with a decrease in the expression levels of FAK, p-FAK, MMP-2, and a decrease in\ the levels of p38-, JNK1/2- and ERK1/2-MAPK pathways as well as inhibiting NF-κB/p65 expression and translocation\ of NF-κB/p65 to the nucleus. We have shown for the first time that the anti-metastatic effects of rhinacanthin-C on\ KKU-M156 cells are mediated via inhibition of the expression of invasion-regulated proteins. Rhinacanthin-C may\ deserve consideration as a potential agent for the treatment of cholangiocarcinoma. Topics: Acanthaceae; Animals; Antineoplastic Agents; Cell Line; Cell Line, Tumor; Cell Movement; Cell Proliferation; Chlorocebus aethiops; Cholangiocarcinoma; Focal Adhesion Kinase 1; Humans; MAP Kinase Signaling System; Matrix Metalloproteinase 2; Mitogen-Activated Protein Kinases; Naphthoquinones; Neoplasm Invasiveness; NF-kappa B; Plant Extracts; Signal Transduction; Urokinase-Type Plasminogen Activator; Vero Cells	2018

Article

Year

Rhinacanthin-C Extracted from Rhinacanthus nasutus (L.) Inhibits Cholangiocarcinoma Cell Migration and Invasion by Decreasing MMP-2, uPA, FAK and MAPK Pathways

Asian Pacific journal of cancer prevention : APJCP, 2018, Dec-25, Volume: 19, Issue:12

Cholangiocarcinoma is a malignant tumor with high metastatic and mortality rates. We investigated the effects\ of rhinacanthin-C on cell proliferation, migration, invasion and the expression of proteins regulating cancer cell\ invasion-regulated proteins in a cholangiocarcinoma (KKU-M156) cell line. Cytotoxicity of rhinacanthin-C was\ determined by the SRB assay. Using wound-migration, chamber-migration and chamber-invasion assays, we assessed\ the effects of rhinacanthin-C against KKU-M156 cells. The activities of matrix metalloproteinases 2 and 9 (MMP-2,\ MMP-9) and urokinase-type plasminogen activator (uPA) were determined using gelatinase and uPA zymography\ assays. The expression of invasion-regulated proteins was investigated using western-blot analysis. After treatment\ with rhinacanthin-C, KKU-M156 cells exhibited antiproliferative effects in a dose-dependent manner with greater\ efficacy than in Vero cells: IC50 values were 1.50 and 2.37 μM, respectively. Rhinacanthin-C significantly inhibited cell\ migration and invasion of KKU-M156 cells in a dose-dependent manner. Consistent with this observation, treatment\ with rhinacanthin-C was associated with a decrease in the expression levels of FAK, p-FAK, MMP-2, and a decrease in\ the levels of p38-, JNK1/2- and ERK1/2-MAPK pathways as well as inhibiting NF-κB/p65 expression and translocation\ of NF-κB/p65 to the nucleus. We have shown for the first time that the anti-metastatic effects of rhinacanthin-C on\ KKU-M156 cells are mediated via inhibition of the expression of invasion-regulated proteins. Rhinacanthin-C may\ deserve consideration as a potential agent for the treatment of cholangiocarcinoma.

Topics: Acanthaceae; Animals; Antineoplastic Agents; Cell Line; Cell Line, Tumor; Cell Movement; Cell Proliferation; Chlorocebus aethiops; Cholangiocarcinoma; Focal Adhesion Kinase 1; Humans; MAP Kinase Signaling System; Matrix Metalloproteinase 2; Mitogen-Activated Protein Kinases; Naphthoquinones; Neoplasm Invasiveness; NF-kappa B; Plant Extracts; Signal Transduction; Urokinase-Type Plasminogen Activator; Vero Cells

2018