rhamnogalacturonan-i and Helicobacter-Infections

rhamnogalacturonan-i has been researched along with Helicobacter-Infections* in 1 studies

Other Studies

1 other study(ies) available for rhamnogalacturonan-i and Helicobacter-Infections

ArticleYear
Acetylated Rhamnogalacturonans from Immature Fruits of Abelmoschus esculentus Inhibit the Adhesion of Helicobacter pylori to Human Gastric Cells by Interaction with Outer Membrane Proteins.
    Molecules (Basel, Switzerland), 2015, Sep-15, Volume: 20, Issue:9

    Polysaccharide containing extracts from immature fruits of okra (Abelmoschus esculentus) are known to exhibit antiadhesive effects against bacterial adhesion of Helicobacter pylori (H. pylori) to stomach tissue. The present study investigates structural and functional features of polymers responsible for this inhibition of bacterial attachment to host cells. Ammonium sulfate precipitation of an aqueous extract yielded two fractions at 60% and 90% saturation with significant antiadhesive effects against H. pylori, strain J99, (FE60% 68% ± 15%; FE90% 75% ± 11% inhibition rates) after preincubation of the bacteria at 1 mg/mL. Sequential extraction of okra fruits yielded hot buffer soluble solids (HBSS) with dose dependent antiadhesive effects against strain J99 and three clinical isolates. Preincubation of H. pylori with HBSS (1 mg/mL) led to reduced binding to 3'-sialyl lactose, sialylated Le(a) and Le(x). A reduction of bacterial binding to ligands complementary to BabA and SabA was observed when bacteria were pretreated with FE90%. Structural analysis of the antiadhesive polysaccharides (molecular weight, monomer composition, linkage analysis, stereochemistry, and acetylation) indicated the presence of acetylated rhamnogalacturonan-I polymers, decorated with short galactose side chains. Deacetylation of HBSS and FE90% resulted in loss of the antiadhesive activity, indicating esterification being a prerequisite for antiadhesive activity.

    Topics: Abelmoschus; Acetylation; Adenocarcinoma; Bacterial Adhesion; Bacterial Outer Membrane Proteins; Fruit; Helicobacter Infections; Helicobacter pylori; Humans; Pectins; Plant Extracts; Polysaccharides; Stomach Neoplasms; Tumor Cells, Cultured

2015