retrorsine and Malaria

retrorsine has been researched along with Malaria* in 1 studies

Other Studies

1 other study(ies) available for retrorsine and Malaria

Article	Year
Expansion, in vivo-ex vivo cycling, and genetic manipulation of primary human hepatocytes. Proceedings of the National Academy of Sciences of the United States of America, 2020, 01-21, Volume: 117, Issue:3 Primary human hepatocytes (PHHs) are an essential tool for modeling drug metabolism and liver disease. However, variable plating efficiencies, short lifespan in culture, and resistance to genetic manipulation have limited their use. Here, we show that the pyrrolizidine alkaloid retrorsine improves PHH repopulation of chimeric mice on average 10-fold and rescues the ability of even poorly plateable donor hepatocytes to provide cells for subsequent ex vivo cultures. These mouse-passaged (mp) PHH cultures overcome the marked donor-to-donor variability of cryopreserved PHH and remain functional for months as demonstrated by metabolic assays and infection with hepatitis B virus and Topics: Animals; Cell Transplantation; Chimera; Disease Models, Animal; Female; Genetic Therapy; Hepatitis B; Hepatitis B virus; Hepatocytes; Homeodomain Proteins; Humans; Hydrolases; Interleukin Receptor Common gamma Subunit; Liver; Liver Diseases; Malaria; Male; Mice; Mice, Inbred NOD; Mice, Knockout; Plasmodium falciparum; Pyrrolizidine Alkaloids	2020

Article

Year

Expansion, in vivo-ex vivo cycling, and genetic manipulation of primary human hepatocytes.

Proceedings of the National Academy of Sciences of the United States of America, 2020, 01-21, Volume: 117, Issue:3

Primary human hepatocytes (PHHs) are an essential tool for modeling drug metabolism and liver disease. However, variable plating efficiencies, short lifespan in culture, and resistance to genetic manipulation have limited their use. Here, we show that the pyrrolizidine alkaloid retrorsine improves PHH repopulation of chimeric mice on average 10-fold and rescues the ability of even poorly plateable donor hepatocytes to provide cells for subsequent ex vivo cultures. These mouse-passaged (mp) PHH cultures overcome the marked donor-to-donor variability of cryopreserved PHH and remain functional for months as demonstrated by metabolic assays and infection with hepatitis B virus and

Topics: Animals; Cell Transplantation; Chimera; Disease Models, Animal; Female; Genetic Therapy; Hepatitis B; Hepatitis B virus; Hepatocytes; Homeodomain Proteins; Humans; Hydrolases; Interleukin Receptor Common gamma Subunit; Liver; Liver Diseases; Malaria; Male; Mice; Mice, Inbred NOD; Mice, Knockout; Plasmodium falciparum; Pyrrolizidine Alkaloids

2020