retinol-acetate and Lung-Neoplasms

Topics: Adjuvants, Immunologic; Animals; Breast Neoplasms; Carcinogens; Cricetinae; Diterpenes; Female; Humans; Keratoacanthoma; Lung Neoplasms; Male; Mice; Neoplasms; Prostatic Neoplasms; Rats; Retinoids; Retinyl Esters; Tretinoin; Vitamin A

An increase of human monoclonal antibody production caused by retinyl acetate and retinoic acid was influenced by the fusion partner rather than the original B lymphocyte used for the human hybridoma generation. Retinoid response of human hybridomas may be at least related to retinoid X receptor-alpha gene expression, which seemed to originate from their fusion partner.

Topics: Adult; Antibodies, Monoclonal; Antibodies, Neoplasm; Antibody Formation; Cell Fusion; Diterpenes; Gene Expression; Humans; Hybridomas; Lung Neoplasms; Receptors, Retinoic Acid; Retinyl Esters; Tretinoin; Tumor Cells, Cultured; Vitamin A

The analysis of clinical and immunologic findings indicate that retinol and decaris influence functional activity of immune cells at stage II of lung cancer irrespective of histological structure. In stage III, retinol induced changes in proportion of lymphocyte subpopulations, more evident in glandular cancer. Both immunomodulators lowered the number of purulent complications after surgical interventions. It is only retinol that influenced metastatic dissemination in lung cancer stage II-III.

Topics: Adenocarcinoma; Adjuvants, Immunologic; Adult; Carcinoma, Squamous Cell; Combined Modality Therapy; Diterpenes; Humans; Levamisole; Lung Neoplasms; Lymphocyte Subsets; Male; Middle Aged; Neoplasm Metastasis; Preoperative Care; Retinyl Esters; Vitamin A

The effect of feeding 0.02% retinyl acetate on the development of cryptogenic neoplasms and the life span of C3H/HeJ (+) mice of both sexes was studied. The survival at 105 weeks was 58% in untreated males and 28% in untreated females vs. 39% in treated males and 14% in treated females. The average weight in treated groups was also 10-15% lower. The incidence (percent) of neoplasm-bearing animals and total neoplasms was 87% and 57, respectively, in female controls vs. 93% and 55 in treated females. In male controls, these values were 57% and 39 compared with 50% and 38 in treated males. In treated animals, there was no reduction in the most common neoplasms, that is, neoplasms of the mammary gland and liver. The numbers of ovarian neoplasms and lung adenomas were slightly lower. Therefore, retinyl acetate exerted, at best, only a slight inhibitory effect on development of some types of cryptogenic neoplasms in mice.

Topics: Animals; Body Weight; Diterpenes; Female; Hemangioma; Liver Neoplasms, Experimental; Longevity; Lung Neoplasms; Male; Mammary Neoplasms, Experimental; Mice; Mice, Inbred C3H; Neoplasms, Experimental; Ovarian Neoplasms; Retinyl Esters; Vitamin A

The ability of retinyl acetate to alter growth, differentiation, and synthesis of mucous glycoproteins in cell lines cloned from an adenocarcinoma (T-8) and a squamous cell carcinoma (1000 WT) was investigated with the use of F344 rats. Growth rate was inhibited approximately 25 and 50% in 6.6 x 10(-6) and 3.3 x 10(-5) M retinyl acetate, respectively. In both cell lines. Cell line T-8 grew mainly as a monolayer, whereas cell line 1000 WT grew as a stratified epithelium. In the presence of both concentrations of retinyl acetate, this stratification was decreased and cells became enlarged and more cuboidal. Retinyl acetate induced the formation of numerous vacuoles and periodic acid-silver methenamine-positive granules in both T-8 and 1000 WT cells. The granules appeared with and without dense cores in electron micrographs. Golgi hypertrophy and increased numbers of microvilli were also evident. After T-8 cells were cultured for 7 days in 6.6 x 10(-6) or 3.3 x 10(-5) M retinyl acetate, [3H]glucosamine incorporation increased 133- to 147-fold and [14C]serine incorporation increased twelvefold to twentyfold in the high-molecular-weight mucous glycoprotein fraction (peak A) from the cell cytosol. In 1000 WT cells, [3H]glucosamine incorporation creased only 4.2- to 7.5-fold, and [14C]serine incorporation increased only 2.6- to 4.6-fold under the same culture conditions. A similar difference in the amount of stimulation was seen for peak A isolated from the secretions. Thus T-8 cells showed a marked increase in the synthesis and secretion of mucins, whereas 1000 WT cells showed a comparatively small but significant increase.

Topics: Animals; Carcinoma, Bronchogenic; Cell Differentiation; Cell Division; Cell Line; Chromatography; Clone Cells; Diterpenes; Glycoproteins; Histocytochemistry; Lung Neoplasms; Neoplasms, Experimental; Rats; Retinyl Esters; Vitamin A