retinamide and Leukemia--Promyelocytic--Acute

retinamide has been researched along with Leukemia--Promyelocytic--Acute* in 1 studies

Other Studies

1 other study(ies) available for retinamide and Leukemia--Promyelocytic--Acute

Article	Year
Early cytoplasmic acidification in retinamide-mediated apoptosis of human promyelocytic leukemia cells. Biochemical and biophysical research communications, 1996, Dec-13, Volume: 229, Issue:2 The retinamide-mediated apoptosis of promyelocytic cells was investigated using pHi- and DNA-sensitive fluorescent probes (BCECF and Hoechst 33342). Acidification and apoptosis were observed during prolonged (0-12 h) exposure to retinamide (1 microM), but were absent in a retinamide-resistant clone. The analysis of experiments performed by simultaneous staining with the two dyes showed that acidification and apoptosis are correlated; the half-time for acidification is about 5 h while that for apoptosis is 80% longer. On the whole, these results suggest that apoptosis is preceded by an early mechanism of acidification in human leukemia cells treated by a retinoid of clinical interest in cancer chemo prevention and therapy. Topics: Apoptosis; Benzimidazoles; Cell Cycle; Cytoplasm; Fluoresceins; Humans; Hydrogen-Ion Concentration; Leukemia, Promyelocytic, Acute; Tretinoin; Tumor Cells, Cultured	1996

Article

Year

Early cytoplasmic acidification in retinamide-mediated apoptosis of human promyelocytic leukemia cells.

Biochemical and biophysical research communications, 1996, Dec-13, Volume: 229, Issue:2

The retinamide-mediated apoptosis of promyelocytic cells was investigated using pHi- and DNA-sensitive fluorescent probes (BCECF and Hoechst 33342). Acidification and apoptosis were observed during prolonged (0-12 h) exposure to retinamide (1 microM), but were absent in a retinamide-resistant clone. The analysis of experiments performed by simultaneous staining with the two dyes showed that acidification and apoptosis are correlated; the half-time for acidification is about 5 h while that for apoptosis is 80% longer. On the whole, these results suggest that apoptosis is preceded by an early mechanism of acidification in human leukemia cells treated by a retinoid of clinical interest in cancer chemo prevention and therapy.

Topics: Apoptosis; Benzimidazoles; Cell Cycle; Cytoplasm; Fluoresceins; Humans; Hydrogen-Ion Concentration; Leukemia, Promyelocytic, Acute; Tretinoin; Tumor Cells, Cultured

1996