retinaldehyde and Retinal-Detachment

Topics: Air; Body Fluids; Drainage; Humans; Injections; Medical Illustration; Recurrence; Reoperation; Retinal Detachment; Retinaldehyde; Silicone Oils

Surgically resected subretinal proliferative tissue (SRP) in proliferative vitreoretinopathy was examined by morphological and immunohistochemical methods. Membranous and strand-like SRPs were observed. Membranous SRP (4 cases) consisted of retinal pigment epithelial cells (RPEs) and a thin layer of extracellular materials. Strand-like SRP (14 cases) had a core, in which the proliferated cells were embedded among extracellular materials. RPEs and glial cells were found on the surface of the core. Immunohistochemical investigation revealed that the predominant cell components of the strand-like SRPs were RPEs and Müller cells, whereas membranous SRPs were composed mainly of RPEs. It was found that the majority of membranous and strand-like SRPs contained types I, III and IV collagens and fibronectin in the extracellular space. Based on the results of this study, a hypothetical process for the formation of SRP tissues was proposed.

Topics: Animals; Carrier Proteins; Extracellular Matrix; Eye Diseases; Glial Fibrillary Acidic Protein; Humans; Immunoenzyme Techniques; Pigment Epithelium of Eye; Rats; Rats, Inbred Strains; Retinal Detachment; Retinal Diseases; Retinaldehyde; Vitreous Body

Retinal detachments were produced in domestic cats by injecting fluid between the retinal pigment epithelium and neural retina. Retinas were allowed to remain detached for 30 or 60 days at which time the animals were killed. Tissue areas from detached and attached retinal regions from the same eye were processed for correlative biochemical and structural analysis, i.e. SDS-PAGE and Western blots of tissue homogenates were correlated with tissue processed for postembedding immunoelectron microscopy. Antibodies to six proteins were used as probes. Glial fibrillary acidic protein in Müller cells has previously been shown to increase after retinal detachment; here we show that vimentin, another intermediate filament protein present in Müller cells, also increases after detachment. In contrast, cellular retinaldehyde binding protein, cellular retinol binding protein, glutamine synthetase, and carbonic anhydrase C--all normally found in Müller cells--decrease after detachment. The results of this study indicate that retinal Müller cells dramatically alter their expression of proteins in response to retinal detachment.

Topics: Animals; Carbonic Anhydrases; Carrier Proteins; Cats; Eye Proteins; Glial Fibrillary Acidic Protein; Neuroglia; Retina; Retinal Detachment; Retinaldehyde; Retinol-Binding Proteins; Retinol-Binding Proteins, Cellular; Vimentin