resolvin-d2 and Asthma

resolvin-d2 has been researched along with Asthma* in 1 studies

Other Studies

1 other study(ies) available for resolvin-d2 and Asthma

ArticleYear
Primary human airway epithelial cell-dependent inhibition of human lung mast cell degranulation.
    PloS one, 2012, Volume: 7, Issue:8

    Chronic mast cell activation is a characteristic feature of asthma. BEAS-2B human airway epithelial cells (AEC) profoundly inhibit both constitutive and IgE-dependent human lung mast cell (HLMC) histamine release. The aim of this study was to examine the regulation of HLMC degranulation by primary AEC from healthy and asthmatic subjects, and investigate further the inhibitory mechanism.. HLMC were co-cultured with both BEAS-2B and primary AEC grown as monolayers or air-liquid interface (ALI) cultures.. Both constitutive and IgE-dependent HLMC histamine release were attenuated by BEAS-2B, primary AEC monolayers and ALI cultures. This occurred in the absence of HLMC-AEC contact indicating the presence of a soluble factor. Unlike healthy ALI AEC, asthmatic ALI-AEC did not significantly reduce constitutive histamine release. AEC inhibitory activity was transferable in primary AEC monolayer supernatant, but less active than with Transwell co-culture, suggesting that the inhibitory factor was labile. The AEC inhibitory effects were attenuated by both AEC wounding and pertussis toxin, indicating the involvement of a G(0)/G(i) receptor coupled mechanism. Solid phase extraction of lipids (<10 kDa) removed the AEC inhibitory activity. The lipid derivatives resolving D1 and D2 and lipoxin A(4) attenuated HLMC histamine release in a dose-dependent fashion but were not detectable in co-culture supernatants.. Primary AEC suppress HLMC constitutive and IgE-dependent histamine secretion through the release of a soluble, labile lipid mediator(s) that signals through the G(0)/G(i) receptor coupled mechanism. Manipulation of this interaction may have a significant therapeutic role in asthma.

    Topics: Asthma; Cell Degranulation; Cells, Cultured; Coculture Techniques; Docosahexaenoic Acids; Epithelial Cells; Histamine Release; Humans; Immunoglobulin E; Inflammation Mediators; Limit of Detection; Lipoxins; Lung; Mast Cells; Paracrine Communication; Pertussis Toxin; Receptors, Prostaglandin E, EP2 Subtype; Respiratory Mucosa; Xanthones

2012