raffinose and Poisoning

Both toxic and physiological substances are adsorbed during an extracorporeal hemoperfusion for the treatment of exogenous and endogenous intoxications. Using a closed circuit in vitro, we perfused one liter saline or fresh human plasma with 4425 mumol creatinine, 4854 mumol and 97,086 mumol barbital-Na, 597 mumol bromthalein, 1942 mumol and 29,126 mumol raffinose, and 200 mumol inulin in different combinations over 70 gm of uncoated charcoal with the following results: 1. The adsorptive capacity of other substances is not influenced by preadsorption of the charcoal with a low or middle molecular weight substance; 2. In the low and middle molecular weight range, there is no competition between two substances in a solution; 3. The simultaneous usage of two substances of middle and high molecular weight, or preadsorption with a high molecular weight substance, reduces the rate of adsorption and the capacity of charcoal for middle molecular weight substances, but not for low molecular weight substances.

Topics: Adsorption; Barbital; Binding, Competitive; Charcoal; Creatinine; Hemoperfusion; Humans; Inulin; Poisoning; Protein Binding; Raffinose; Sulfobromophthalein; Surface Properties