raffinose and Colonic-Neoplasms

In this experimental model, taurine administered during hypoxia markedly reduced the cell damage due to O2 deficiency, and the beneficial effect outlasted the period of reoxygenation. The mechanisms for the improved survival rates are postulated to be a reduced osmoregulatory disturbance of cellular integrity, improved Ca2+ homeostasis and induction of accelerated cellular growth processes. In our simplified cell culture model the UW solution seems to be the most appropriate solution for the cold (hypoxic) preservation of human colon cells. We conclude, that within this experimental model and under these experimental conditions, taurine supplementation of the conventionally used preservation solutions improved the solutions markedly. Considering our previous studies, taurine seems to be a potent endogenous protective agent against cellular deterioration due to hypoxia and reoxygenation.

Topics: Adenosine; Aerobiosis; Allopurinol; Cell Hypoxia; Cell Line; Cell Survival; Colon; Colonic Neoplasms; Glucose; Glutathione; Humans; Hypertonic Solutions; Insulin; Mannitol; Organ Preservation Solutions; Potassium Chloride; Procaine; Raffinose; Taurine; Tissue Preservation

Intestinal alkaline phosphatase (AP) is secreted by Caco-2 cells bound to surfactant-like particles (SLP), which can be localized by electron microscopy to the basolateral space and the intestinal lumen, especially over tight junctions. To investigate the hypothesis that SLP are secreted basolaterally and enter the lumen through the tight junction, Caco-2 cells were incubated with agents known to increase permeability at tight junctions. Cytochalasin D and phorbol 12-myristate 13-acetate increased Caco-2 cell monolayer permeability and the appearance of particles in apical medium two- to threefold, as monitored by mannitol movement and AP activity, respectively. Blocking the apical secretory pathway by nocodazole or colchicine had no effect on either parameter. Estimation of SLP content demonstrated an increase in apical media particles similar to that determined by AP activity. Quantitative image analysis established that apical SLP content increased 4-10 times, whereas total cell particle content remained unchanged. These data indicate that SLP may be secreted initially into the basolateral space and then transported to the intestinal lumen through the tight junctions.

Topics: Adenocarcinoma; Alkaline Phosphatase; Animals; Antibodies; Cell Line; Cell Membrane; Cell Membrane Permeability; Colonic Neoplasms; Cytochalasin D; Humans; Hypertonic Solutions; Intercellular Junctions; Intestine, Small; Microscopy, Electron; Organelles; Rabbits; Raffinose; Sucrose; Surface-Active Agents; Tetradecanoylphorbol Acetate; Time Factors; Tumor Cells, Cultured

Topics: Adenoma, Bile Duct; Adenosine; Adult; Allopurinol; Bile Duct Neoplasms; Carcinoma, Hepatocellular; Colonic Neoplasms; Duodenal Neoplasms; Duodenum; Female; Glutathione; Humans; Insulin; Intestine, Small; Liver Neoplasms; Liver Transplantation; Male; Middle Aged; Organ Preservation; Organ Preservation Solutions; Pancreas Transplantation; Pancreatic Neoplasms; Raffinose; Solutions