rabdosiin and Proteinuria

During an investigation of plant cell cultures that might be useful in the treatment of renal disorders, we established a vigorously-growing E-4 callus culture of Eritrichium sericeum that produced large amounts of caffeic acid metabolites, (-)-rabdosiin (1.8% dry wt) and rosmarinic acid (4.6% dry wt). Elicitation of the calli by methyl jasmonate induced a 38% increase in total polyphenol production. The most efficient method of eliciting (-)-rabdosiin biosynthesis was through the treatment of E-4 calli with cuprum glycerate, which induced an increase in (-)-rabdosiin production of as much as 4.1% dry wt. Oral administration of E-4 callus biomass (100 mg/kg/d for 30 d) to rats with induced Masugi-nephritis caused an increase in diuresis and lowered creatinine excretion and proteinuria levels as compared with Masugi-nephritis untreated rats. While all of the Masugi-nephritis untreated rats began to suffer, near a quarter of the E-4 treated rats remained in good health. This result indicates that the E-4 culture has the potential to alleviate the symptoms associated with nephritis.

Topics: Acetates; Animals; Biomass; Boraginaceae; Caffeic Acids; Cells, Cultured; Cinnamates; Copper; Creatinine; Cyclopentanes; Depsides; Diuresis; Glyceric Acids; Kinetics; Lignans; Molecular Structure; Nephritis; Oxylipins; Phytotherapy; Plant Growth Regulators; Proteinuria; Random Allocation; Rats; Rats, Sprague-Dawley; Rosmarinic Acid