quercetagetin and Nasopharyngeal-Neoplasms

To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma (NPC) cells.. Pim-1 expressions in NPC cell lines CNE1, CNE1-GL, CNE-2Z and C666-1 were examined by RT-PCR, western blotting and immunoflucesence, respectively. After CNE1, CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor, quercetagetin, the cell viability, colony formation rate and migration ability were analyzed.. Pim-1 expression was negative in well-differentiated CNE1 cells, whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells. Interestingly, CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1. Treatment of CNE1-GL and C666-1 cells with quercetagetin significantly decreased the cell viability, colony formation rate and migration ability but not the CNE1 cells.. These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration, and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.

Topics: Antineoplastic Agents; Biomarkers, Tumor; Blotting, Western; Carcinoma; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cell Survival; Chromones; Flavones; Fluorescent Antibody Technique, Indirect; Humans; Nasopharyngeal Carcinoma; Nasopharyngeal Neoplasms; Proto-Oncogene Mas; Proto-Oncogene Proteins c-pim-1; Reverse Transcriptase Polymerase Chain Reaction; Tumor Stem Cell Assay