quercetagetin and Inflammation

One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is. We investigated the effects of luteolin, quercetin, genistein and quercetagetin on cardiomyoblasts treated with LPS alone or in combination with following inhibitors p38 (SB203580), ERK (PD98059), JNK (SP600125) and PKC (Calphostin C) for 1 h. The kinase activation and COX-2 expression levels were determined at the gene and protein levels.. These flavonoids are considered to inhibit the activation of mitogen-activated protein kinase (MAPK) and the degradation of inhibitor of kappa B-alpha (IκB-α). They also play a role in COX-2 expression.. We conclude that the tested flavonoids inhibit inflammatory responses induced by LPS in H9c2 cells.

Topics: Animals; Chromones; Cyclooxygenase 2; Flavones; Flavonoids; Gene Expression Regulation; Genistein; Inflammation; Lipopolysaccharides; Luteolin; Mitogen-Activated Protein Kinases; Myocytes, Cardiac; Porphyromonas gingivalis; Quercetin; Rats

Six flavonoid derivatives were tested for their influence on Naja naja and human recombinant synovial phospholipase A2. They showed a selectivity for the last enzyme with IC50 = 14.3, 17.6, 12.2 and 28.2 microM for quercetagetin, kaempferol-3-O-galactoside, scutellarein and scutellarein-7-O-glucuronide, respectively, while reduced effects were observed for hispidulin and hibifolin. After topical application all the flavonoids inhibited 12-O-tetradecanoylphorbol-13-acetate-induced ear oedema in mice with a potency comparable to that of indomethacin and they were also able to inhibit carrageenan-induced mouse paw oedema at a dose of 150 mg/kg p.o. The blockade of the free hydroxyl at C-7 or C-6 reduced the anti-inflammatory activity and also the inhibitory effect on human recombinant synovial phospholipase A2. These results are in accordance with the notion that group II phospholipases A2 may play a role in experimental inflammation, although several mechanisms seems to be involved in the anti-inflammatory effect of this group of flavonoids.

Topics: Animals; Chromones; Edema; Flavanones; Flavones; Flavonoids; Galactosides; Humans; Inflammation; Kaempferols; Male; Mice; Phospholipases A; Phospholipases A2; Quercetin; Recombinant Proteins; Snakes; Tetradecanoylphorbol Acetate