pyrophosphate and Wilms-Tumor

Osteoscintigraphy and dynamic scintigraphy of the kidneys with 99mTc-pyrophosphate were used for investigation of 24 pediatric patients with tumorous involvement of the retroperitoneal space. Bone metastases were detected by osteoscintigraphy in 8 patients, pathological changes of the kidneys and urinary tract in 9 patients. Dynamic investigation of the kidneys with 99mTc-pyrophosphate performed in the first 20 min. after the drug injection showed disorders of renal evacuatory function of a varied degree of expression in all 24 patients. Dynamic investigation of the kidneys with 99mTc-pyrophosphate was not accompanied by an increase in radiation exposures therefore it was recommended at the first stage of radionuclide investigation of children with tumorous involvement of the retroperitoneal space followed by osteoscintigraphy.

Topics: Adult; Bone Neoplasms; Child; Child, Preschool; Diphosphates; Hodgkin Disease; Humans; Infant; Kidney Neoplasms; Neuroblastoma; Radioisotope Renography; Retroperitoneal Neoplasms; Technetium; Technetium Tc 99m Pyrophosphate; Wilms Tumor

In static scintigraphy with 99m Tc-pertechnetate of primary lesions of the retroperitoneal space in children 4 types of scintigraphic pictures were singled out for differential diagnosis of Wilms' tumors, avascular benign lesions of the retroperitoneal space and neurogenic retroperitoneal tumors. The sensitivity of diagnosis of Wilms' tumor was 92.6%, specificity 90.9%. The most objective and complete information on the kidney involvement in tumor process could be obtained in static scintigraphy with 99m Tc-pertechnetate combined with scintigraphy with 99m Tc-gluconate or 99m Tc-pyrophosphate. The sensitivity of diagnosis of secondary lesions of the lymph nodes of the retroperitoneal space was 76.8%.

Topics: Adolescent; Child; Child, Preschool; Diphosphates; Gluconates; Humans; Infant; Kidney Neoplasms; Organotechnetium Compounds; Radionuclide Imaging; Retroperitoneal Neoplasms; Sodium Pertechnetate Tc 99m; Technetium; Technetium Tc 99m Pyrophosphate; Wilms Tumor

Purified preparations of the parvovirus, Kilham rat virus, have associated with them a protein with DNA polymerase activity. The enzyme has been separated from the other two or three viral proteins and purified 63-fold. The viral associated enzyme was found in a single peak of DNA polymerase activity after chromatography on DEAE-cellulose, DNA-cellulose, and phosphocellulose columns. It shares some properties in common with the host cellular DNA polymerases, described in the preceding paper (Salzman, L.A., and McKerlie, L. (1975) J. Biol. Chem. 250, 5589-5595), but also has some important distinguishing characteristics. The Kilham rat virus-associated DNA polymerase has increased enzyme activity in the presence of 0.02 M KCl and has a strong preference for a synthetic DNA polymer containing deoxyadenylate and deoxythymidylate. The enzyme has a molecular weight of approximately 75,000 plus or minus 3,000 and appears to contain endonuclease activity.

Topics: Animals; Cell Line; Centrifugation, Density Gradient; Chloromercuribenzoates; Chromatography, Affinity; Chromatography, DEAE-Cellulose; Chromatography, Gel; Diphosphates; DNA Nucleotidyltransferases; DNA, Viral; Ethidium; Exonucleases; Hydrogen-Ion Concentration; Magnesium; Manganese; Molecular Weight; Nucleotides; Parvoviridae; Potassium Chloride; Rats; Simian virus 40; Sodium Chloride; Templates, Genetic; Tritium; Wilms Tumor

We have examined the DNA polymerases found in a rat nephroma cell line. Using DEAE- and DNA-cellulose chromatography, we have found two major cytoplasmic DNA polymerases and one major and three minor DNA polymerases from the nucleus. The enzymes were all purified, characterized, and distinguished from each other by several criteria. The enzyme require, for maximal activity, a natural or synthetic double-stranded DNA, four deoxynucleoside, triphosphates, and magnesium. They are inhibited to varying degrees by sodium pyrophosphate, ethidium bromide, and rho-chloromercuribenzoate.

Topics: Animals; Cell Line; Cell Nucleus; Chloromercuribenzoates; Chromatography, Affinity; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cytoplasm; Diphosphates; DNA Nucleotidyltransferases; DNA, Viral; Endonucleases; Ethidium; Exonucleases; Hydrogen-Ion Concentration; Kinetics; Magnesium; Molecular Weight; Potassium Chloride; Rats; Sodium Chloride; Templates, Genetic; Thymidine; Tritium; Wilms Tumor