pyrophosphate and Pleurisy

We studied the sequential cellular events that occur during nonspecific pleurisy induced by a nondiffusible, nonantigenic and endotoxin-free irritant, calcium pyrophosphate (CaPP). The study was conducted over 10 days and concerned not only the pleural cavity but also the peritoneal cavity and the blood. After injection of CaPP, the first cellular event in the pleural space was a "leukocyte disappearance reaction," followed by an important increase in the polymorphonuclear leukocytes (PMN) and then by an increase in the macrophages. This phase is also characterized by the appearance of submesothelial nodules composed of macrophages and numerous polymorphonuclear cells. All the cell populations returned to their normal value on day 10, although granulomatous submesothelial nodules were present. In the blood, the principal observation was an increased PMN count associated with a decreased lymphocyte count at 4 h. In the peritoneal cavity, it was remarkable that, although functional modifications have been reported in the same model, the different leukocyte populations did not vary during the reaction.

Topics: Animals; Blood Cell Count; Blood Cells; Bronchoalveolar Lavage Fluid; Calcium Pyrophosphate; Diphosphates; Male; Pleura; Pleurisy; Rats; Rats, Inbred Strains

1 The effects of indomethacin, dexamethasone and colchicine on cyclic adenosine 3', 5'-monophosphate (cyclic AMP) concentration in leucocytes during a crystal-induced pleurisy in rats were studied. 2 Each of the drugs significantly increased leucocyte cyclic AMP content within 3 h of the injection of crystals. 3 By 6 h, leucocyte cyclic AMP levels returning toward control levels and could not be sustained at the higher level by an additional administration of the respective anti-inflammatory drug.

Topics: Animals; Anti-Inflammatory Agents; Colchicine; Cyclic AMP; Dexamethasone; Diphosphates; Indomethacin; Leukocytes; Male; Pleurisy; Rats; Time Factors

The changes in leucocyte concentrations of cyclic AMP and cyclic GMP were monitored during pyrophosphate-induced pleurisy in rats. A classical 'Yin Yang' relationship was demonstrated.

Topics: Animals; Cyclic AMP; Cyclic GMP; Diphosphates; Inflammation; Male; Pleurisy; Rats; Time Factors

A model is described of acute inflammation in the pleural cavity of rats using calcium pyrophosphate as the irritant. This model would seem to simulate the pseudogout syndrome. It has been shown to be acute in onset, dominated by polymorphonuclear cells, complement independent. The advantage of the model is that volume of exudate, numbers and types of cells may be quantitated. Prostaglandins and cyclic AMP have been measured in the migrating cells. The significance of these findings has been discussed.

Topics: Animals; Calcium Phosphates; Cyclic AMP; Diphosphates; Disease Models, Animal; Indomethacin; Inflammation; Male; Pleural Effusion; Pleurisy; Prostaglandins E; Prostaglandins F; Rats; Snake Venoms

Topics: Animals; Arthritis; Arthus Reaction; Calcium; Carrageenan; Chondrocalcinosis; Diphosphates; Disease Models, Animal; Guinea Pigs; Immunity, Cellular; Kinetics; Macrophages; Phosphorus; Pleurisy; Rats