pyrophosphate and Leukemia--Erythroblastic--Acute

Topics: Adult; Brain Diseases; Burns, Electric; Cardiovascular Diseases; Digestive System Diseases; Diphosphates; Diphosphonates; Female; Humans; Leukemia, Erythroblastic, Acute; Lung Diseases; Male; Middle Aged; Polyphosphates; Radionuclide Imaging; Splenic Diseases; Technetium; Technetium Compounds; Technetium Tc 99m Pyrophosphate; Urologic Diseases

Poly(A) polymerase activity was studied in lysates of cultured murine erythroleukemic cells (Friend cells). Incorporation of ATP into acid-precipitable products is dependendent on the presence of Mn2+ or Mg2+ and of an RNA primer. The reaction is specific for ATP as the substrate (KM=290 290 micron, it is not inhibited by actinomycin D and only slightly interferred with by ethidium bromide. Cordycepin 5'-triphosphate and sodium pyrophosphate inhibit the enzyme activity. The chain length of the products of the reaction is dependent on the primer concentration and reaches up to 30 nucleotides. Poly(A) polymerase activity is low in resting (G1 phase) cells 75 nmol ATP incorporated/h per 10(6) cells) and increases to a level about twice as high in early S phase of the cell cycle. A possible model for regulation of enzyme activity is discussed. Polymerase activity in the early phase of erythropoietic differentiation of the cells induced by butyric acid does not show any difference in comparison to untreated controls. A decrease in enzyme activity to levels characteristic for cells in G1 phase accompanies shutdown of cell growth in the course of the ongoing differentiation. Analysis of the DNA content of the cells revealed that erythropoietic differentiation of Friend cells induced by butyric acid is characterized by arrest of the cells in G1 phase of the cell cycle. Poly(A) polymerase activity in erythroleukemic cells is thus controlled only by the phase of the cell cycle; it is not affected by changes in gene expression during erythroid differentiation.

Topics: Adenosine Triphosphate; Animals; Cell Cycle; Cell Differentiation; Deoxyadenosines; Diphosphates; DNA; Erythropoiesis; Leukemia, Erythroblastic, Acute; Mice; Nucleotidyltransferases; Polynucleotide Adenylyltransferase; RNA; Spleen