pyrophosphate and Acromegaly

Topics: Acromegaly; Acute Disease; Aged; Arthritis, Rheumatoid; Bursitis; Calcium Phosphates; Chondrocalcinosis; Chronic Disease; Colitis, Ulcerative; Crystallization; Diphosphates; Female; Gout; Humans; Knee Joint; Male; Middle Aged; Osteoarthritis; Pyrophosphatases; Radioisotope Dilution Technique; Synovial Fluid

A rapid and relatively simple method for measurement of inorganic pyrophosphate (PPi) in biological samples has been described. The mean +/-SEM of plasma samples from 94 normal subjects was 1.8+/-0.06 muM, giving a normal range (99% confidence limits) of 0.16 - 3.40 mumol/liter. Analysis of 17 plasma samples in duplicate showed a standard deviation of 0.18, giving a 99% probability that a single determination of plasma PPi would be +/-0.68 muM of the true value. The mean PPi levels in plasma from subjects with osteoarthritis, pseudogout, acromegaly, and uremia were significantly greater than the normal mean (P < 0.01). Samples from rheumatoid arthritis showed PPi levels distributed about a mean identical to the normal mean. Plasma inorganic orthophosphate levels correlated positively with PPi levels in samples from normal subjects and in samples from patients with osteoarthritis, pseudogout, and uremia, but not with acromegaly. This correlation was statistically significant only in the normal samples and in those from patients with osteoarthritis.

Topics: Acromegaly; Adolescent; Adult; Aged; Arthritis, Rheumatoid; Body Fluids; Child; Child, Preschool; Chondrocalcinosis; Colorimetry; Diphosphates; Female; Humans; Infant; Infant, Newborn; Male; Middle Aged; Osteoarthritis; Phosphorus Isotopes; Pyrophosphatases; Uremia

Topics: Acromegaly; Adult; Calcinosis; Calcium Phosphates; Cartilage Diseases; Diagnosis, Differential; Diphosphates; Gout; Hemochromatosis; Humans; Hyperparathyroidism; Joints; Male; Microscopy, Polarization; Middle Aged; Radiography; Terminology as Topic