pyrimidinones and Spherocytosis--Hereditary

The fluorescent probe merocyanine 540 does not stain the plasma membrane of normal human or murine erythrocytes, nor of genetically abnormal human spherocytic erythrocytes. It does, however, stain erythrocyte membranes in several systems in which the underlying spectrin network is altered or missing. Because of the greater affinity of merocyanine 540 for fluid--phase lipid bilayers, these results suggest that the external leaflet of erythrocyte membranes becomes more disordered upon alteration or loss of the internal spectrin network. Analysis of the transbilayer arrangement of membrane phospholipids by digestion with phospholipase A2 suggests that lipid compositional asymmetry of the erythrocyte membrane is responsible for a phase-state asymmetry between the two lipid leaflets, and that spectrin is required to maintain this asymmetry and the gel-like state of the external leaflet.

Topics: Animals; Erythrocyte Aging; Erythrocyte Membrane; Erythrocytes; Humans; Membrane Fluidity; Membrane Lipids; Membrane Proteins; Phospholipases A; Phospholipases A2; Pyrimidinones; Spectrin; Spherocytosis, Hereditary; Tetrathionic Acid