pyridostatin and Virus-Diseases

Herein, we report, for the first time, the screening of several ligands in terms of their ability to bind and stabilize G-quadruplexes (G4) found in seven human Papillomavirus (HPV) genomes. Using a variety of biophysical assays, HPV G-quadruplexes were shown to possess a high degree of structural polymorphism upon ligand binding, which may have an impact on transcription, replication, and viral protein production. A sequence found in high-risk HPV16 genotype folds into multiple non-canonical DNA structures; it was converted into a major G4 conformation upon interaction with a well-characterized highly selective G4 ligand, PhenDC3, which may have an impact on the viral infection. Likewise, HPV57 and 58, which fold into multiple G4 structures, were found to form single stable complexes in the presence of two other G4 ligands, C

Topics: Aminoquinolines; Complement C8; DNA-Binding Proteins; G-Quadruplexes; Genome, Viral; Genotype; Human papillomavirus 16; Human papillomavirus 18; Humans; Ligands; Molecular Targeted Therapy; Nucleic Acid Conformation; Picolinic Acids; Virus Diseases