pyoverdin and Pneumonia

The increasing prevalence of antibiotic resistance in Pseudomonas aeruginosa has created an urgent need for suitable therapy. This study explored the pairing of doxycycline with other antipseudomonal antibiotics, and found that polymyxin B in combination with doxycycline had a synergistic effect against clinical strains of P. aeruginosa. This synergistic combination was studied by checkerboard assays and time-kill curve analysis. Further, in-vitro biofilm disruption, pyoverdine inhibition assays were performed. The efficacy of polymyxin B-doxycycline in combination, administered by inhalation, was evaluated using a mouse model of acute pneumonia. The combination was found to have a synergistic effect in both in-vitro and in-vivo studies. The combination decreased biofilms of P. aeruginosa and reduced the level of pyoverdine, an important siderophore of P. aeruginosa. In addition, the combination decreased the P. aeruginosa population by 3 log

Topics: Animals; Anti-Bacterial Agents; Biofilms; Disease Models, Animal; Doxycycline; Drug Resistance, Multiple, Bacterial; Drug Synergism; Drug Therapy, Combination; Lung; Mice; Microbial Sensitivity Tests; Oligopeptides; Pneumonia; Polymyxin B; Pseudomonas aeruginosa; Pseudomonas Infections; Respiratory Function Tests