punicalagin and Asthma

To explore the therapeutic effect and mechanism of punicalagin on bronchial asthma in the elderly.. Peripheral venous blood was collected from healthy people and elderly patients with bronchial asthma. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured. PBMCs in the patient group were treated with different concentrations (0, 50, 100, and 200 mg/L) of punicalagin (PUN). MTT assay was used to detect cell activity, ELISA was used to detect the levels of IFN-. MTT results showed that 50-200 mg/L PUN had no cytotoxicity to PBMCs within 24 h. ELISA results showed that the levels of IFN-. PUN can inhibit Th2 differentiation and regulate Th1/Th2 balance by regulating IL-4/STAT6 and Jagged1/Notch pathways to alleviate the progress of bronchial asthma in the elderly.

Topics: Aged; Asthma; Humans; Hydrolyzable Tannins; Interleukin-2; Interleukin-4; Interleukin-5; Jagged-1 Protein; Leukocytes, Mononuclear; Receptors, Notch; STAT6 Transcription Factor

Topics: Animals; Anti-Asthmatic Agents; Asthma; Disease Models, Animal; Female; GATA3 Transcription Factor; Inflammation; Interleukin-4; Lung; Mice; Mice, Inbred BALB C; STAT6 Transcription Factor; Th2 Cells