psd-502 and Inflammation

We assessed the effects of prior application of a eutectic mixture of local anesthetics (EMLA) on the appearance of dyschromia at the site of superficial electrodesiccation in an observer-blind, case-control study in 60 patients. Thirty subjects each were assigned to Groups A and B; both groups underwent radiofrequency (RFC) ablation for facial dermatosis papulosa nigrans (DPN). Group A received RFC ablation with prior application of EMLA, whereas Group B did not. No significant difference was observed in the dyschromia between both groups. EMLA cream was well tolerated by the study participants. (

Topics: Adult; Anesthetics, Local; Case-Control Studies; Double-Blind Method; Electrocoagulation; Facial Dermatoses; Female; Humans; Hyperpigmentation; Inflammation; Lidocaine, Prilocaine Drug Combination; Male; Middle Aged

Topics: Anesthetics, Local; Double-Blind Method; Humans; Hypersensitivity; Inflammation; Lidocaine; Lidocaine, Prilocaine Drug Combination; Prilocaine; Skin; Skin Tests

The aim of this present study was to evaluate the irritative potential of 2 topical anesthetics used in intrapocket anesthesia for periodontal scaling/root planing when applied in subcutaneous tissue of rats. Sixty animals were divided into 4 groups: group 1, saline solution (control); group 2, poloxamer gel (thermosetting vehicle); group 3, lidocaine and prilocaine poloxamer thermosetting gel; group 4: EMLA, a lidocaine and prilocaine dermatological cream. Injections of 2% Evans blue were administrated intravenously into the lateral caudal vein. In order to analyze vascular permeability, the tested substances were injected intradermally. The rats were sacrificed 3, 6, and 9 hours after injection of the substances. The dorsal skin was dissected and removed. The vascular permeability was evaluated by the measurement of area of dye extravasation and the dye was subsequently extracted after immersion in formamide. Statistical analyses were made by ANOVA with Bonferroni's post hoc test and Pearson correlation. The 2 methods to analyze the exudative phase of the inflammatory process showed statistically significant difference among the groups and periods of evaluation (P < .05). Both methods had a significant correlation (P < .0001). Under the tested conditions, the anesthetic agents showed mild initial inflammatory response when implanted in subcutaneous connective tissue.

Topics: Anesthetics, Combined; Anesthetics, Local; Animals; Biocompatible Materials; Capillary Permeability; Dental Scaling; Drug Combinations; Exudates and Transudates; Inflammation; Injections, Intradermal; Lidocaine; Lidocaine, Prilocaine Drug Combination; Male; Prilocaine; Rats; Rats, Wistar; Subcutaneous Tissue

Most studies investigating the pathophysiological processes taking place inside an experimental burn wound use in vitro techniques, which only allow for fragmented measurements of the actual and complex processes occurring inside a burn wound in vivo. In the present study, which used a recently developed in vivo technique in the rat, a full-thickness burn was induced and resulted in the formation of a subcutaneous gelatinous edema with distinct borders to the surrounding connective tissue and free communication with the systemic circulation allowing it to be easily separated for further analysis. In the present study, we investigated the effects of topical local anaesthetics (EMLA) on the inflammatory cascade of a burn wound in vivo. Results showed significantly higher myeloperoxidase (MPO) levels in EMLA-treated burned animals (P<0.01) versus placebo-treated burned controls. EMLA treatment induced a significant inhibition of the synthesis of leukotrien B(4) (LTB(4)) (P<0.001), prostaglandin E(1) (PGE(1)) (P<0.001), prostaglandin E(2) (PGE(2)) (P<0.001) and thromboxane B(2) (TXB(2)) (P<0.001) versus control, while free radical formation did not differ significantly between EMLA-treated and control animals. In conclusion, topical local anaesthetics significantly inhibit the release of several mediators known to take important part in the pathophysiological events ensuing a burn injury, such as activation of pain mechanisms (PGE), oedema formation (LTB), and postburn ischemia (TXB). The increased numbers of leukocytes (MPO) in the burn wound induced by topical local anaesthetic treatment could suggest increased influx and/or increased viability of leukocytes postburn.

Topics: Administration, Topical; Anesthetics, Local; Animals; Burns; Free Radicals; Inflammation; Leukotriene B4; Lidocaine; Lidocaine, Prilocaine Drug Combination; Male; Models, Animal; Peroxidase; Prilocaine; Prostaglandins E; Rats; Rats, Sprague-Dawley; Thromboxane B2

Infection around implants is the most frequently described problem during limb lengthening. Fast elimination of the inflammatory response is crucial for intensive mobilization and weight bearing of the extremity being elongated. Prior to the unloading incision of the skin stretched around the implant EMLA ointment was used repeatedly in 12 children. EMLA was applied 1-2 hours prior to the procedure and covered with an occlusive dressing. All skin incisions, also within inflamed areas were painless. The management described eliminates the need for general anesthesia and thus for hospitalization, lowers treatment costs and limits child's anxiety.

Topics: Adolescent; Anesthesia, Local; Anesthetics, Local; Bone Lengthening; Child; Child, Preschool; Cost Control; Drug Combinations; Female; Humans; Inflammation; Lidocaine; Lidocaine, Prilocaine Drug Combination; Male; Occlusive Dressings; Poland; Prilocaine; Prostheses and Implants; Weight-Bearing