prostaglandin-d2 and Renal-Insufficiency--Chronic

Lipid-metabolizing enzymes and their metabolites affect inflammation and fibrosis, but their roles in chronic kidney disease (CKD) have not been completely understood.. To clarify their role in CKD, we measured the mRNA levels of major lipid-metabolizing enzymes in 5/6 nephrectomized (Nx) kidneys of C57BL/6 J mice. Mediator lipidomics was performed to reveal lipid profiles of CKD kidneys.. In 5/6 Nx kidneys, both mRNA and protein levels of Alox15 were higher when compared with those in sham kidneys. With respect to in situ hybridization, the mRNA level of Alox15 was higher in renal tubules of 5/6 Nx kidneys. To examine the role of Alox15 in CKD pathogenesis, we performed 5/6 Nx on Alox15. Increased PGD

Topics: Actins; Animals; Arachidonate 12-Lipoxygenase; Arachidonate 15-Lipoxygenase; Cell Line; Collagen Type I; Collagen Type I, alpha 1 Chain; Fibrosis; Humans; Intramolecular Oxidoreductases; Kidney; Kidney Tubules, Proximal; Lipid Metabolism; Lipocalins; Male; Mice, Inbred C57BL; Nephrectomy; Prostaglandin D2; Renal Insufficiency, Chronic; RNA, Messenger