prostaglandin-d2 and Cartilage-Diseases

Aiming to delineate novel neuro-immune mechanisms for NGF/TrkA signalling in osteoarthritis (OA) pain, we evaluated inflammatory changes in the knee joints following injection of monoiodoacetate (MIA) in mice carrying a TrkA receptor mutation (P782S; TrkA KI mice).. In behavioural studies we monitored mechanical hypersensitivity following intra-articular MIA and oral prostaglandin D. In TrkA KI mice we observed rapid development of mechanical hypersensitivity and amplification of dorsal horn neurons and microglia activation 7 days after MIA. In TrkA KI knee joints we detected significant leukocyte infiltration and mast cells located in the vicinity of synovial nociceptive fibres. We demonstrated that mast cells exposure to NGF results in up-regulation of COX-2 and increase of PGD. Using the TrkA KI mouse model, we delineated a novel neuro-immune pathway and suggest that NGF-induced production of PGD

Topics: Animals; Arthritis, Experimental; Cartilage Diseases; Cartilage, Articular; Cyclooxygenase 2; Disease Models, Animal; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Enzyme Inhibitors; Female; Injections, Intra-Articular; Intramolecular Oxidoreductases; Iodoacetic Acid; Lipocalins; Macrophages; Male; Mast Cells; Mice, Inbred C57BL; Osteoarthritis, Knee; Prostaglandin D2; Receptor, trkA; Stifle; T-Lymphocytes; Up-Regulation