propargylcholine and Disease-Models--Animal

The lack of biomarkers is a major obstacle for investigating myelin repair. We used metabolic incorporation of the choline analog - propargyl-choline (P-Cho) to label and visualize newly synthesized myelin in the CNS of mice induced with experimental autoimmune encephalomyelitis (EAE). We further developed unbiased colocalization analysis to quantify P-Cho incorporation specifically into the myelin. Our findings indicate that P-Cho injection to mice recovering from EAE, either spontaneously or following glatiramer acetate treatment, results in significant elevation of its incorporation into the myelin, offering a novel strategy for assessing remyelination in animal models and the remyelination potential of candidate drugs.

Topics: Alkynes; Animals; Cell Nucleolus; Choline; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental; Glatiramer Acetate; Immunosuppressive Agents; Magnetic Resonance Imaging; Mice; Mice, Inbred BALB C; Microscopy, Immunoelectron; Myelin Basic Protein; Myelin Proteolipid Protein; Myelin Sheath; Peptide Fragments; Recovery of Function; Spinal Cord