prodigiosin and Inflammation

Topics: Animals; Anti-Bacterial Agents; Antigen-Antibody Reactions; Antineoplastic Agents; Bronchitis; Cholecystitis; Colitis; Drug-Related Side Effects and Adverse Reactions; Female; Genital Diseases, Female; Hepatitis A; Humans; Inflammation; Influenza, Human; Neoplasms, Experimental; Occupational Diseases; Periodontal Diseases; Phagocytosis; Pituitary-Adrenal System; Pregnancy; Pregnancy Complications, Infectious; Prodigiosin; Psoriasis; Pyelonephritis; Radiation-Protective Agents; Surgical Wound Infection; Tonsillitis; Virus Diseases

Prodigiosin (PG) is a secondary metabolite of microorganisms with anticancer, antimalarial, antibacterial and immunomodulatory effects. However, the modulatory effects on gut microbiome and intestinal immune microenvironment have never been explored in the ulcerative colitis (UC) mice model. In this study, 2.5% dextran sulfate sodium (DSS) induced UC mice model was constructed to investigate the effects of PG derived from a chromium-resistant Serratia sp. on the intestinal flora and inflammatory response. The results showed that prodigiosin administration attenuated the DSS-induced UC symptoms, including preventing the reduction of colonic length and DSS-induced mortality. Furthermore, prodigiosin ameliorated the DSS-induced gut microbiota community dysbiosis by restoring the abundance of Bacteroidota. At the genus level, the declined abundance of Bifidobacterium, Allobaculum and Akkermannia in UC mice was elevated by the treatment of PG. Pathological results by H&E staining showed that PG prevented the appearance of distortion and atrophy of crypt and neutrophil infiltration in a dose-dependent manner. RT-PCR revealed that the expression levels of the inflammatory factors IL-1β, IL-6 and IL-10 were significantly suppressed, and the expression of the intestinal tight junction protein Claudin-1, Occludin and ZO-1 were upregulted in PG-treated UC mice. Conclusively, our results revealed that prodigiosin effectively prevented inflammatory response and protected intestinal barrier integrity of DSS-induced colitis mice via modulating gut microbiota community structure, suppressing inflammatory factors' expression, and accelerating the expression of intestinal tight junction protein. These results will provide new insights into the interaction of prodigiosin with intestinal microbiota homeostasis and its application in clinical against inflammatory bowel disease.

Topics: Animals; Colitis; Colitis, Ulcerative; Colon; Dextran Sulfate; Disease Models, Animal; Gastrointestinal Microbiome; Homeostasis; Inflammation; Mice; Mice, Inbred C57BL; Prodigiosin; Tight Junction Proteins

Prodigiosins, which are natural tripyrrole red pigments and synthetic derivatives, reportedly have multiple biological effects mainly on various types of cancer cells. However, the effects of bacterial prodigiosin on non-cancerous HaCaT human skin keratinocytes have not been reported. Therefore, the present study aimed to investigate the functional activities of prodigiosin derived from cultures of the bacterium

Topics: Cell Proliferation; Cell Survival; Collagen; Cytokines; Gammaproteobacteria; HaCaT Cells; Humans; Inflammation; Keratinocytes; Matrix Metalloproteinase 9; Prodigiosin; Reactive Oxygen Species; Skin Aging; Ultraviolet Rays

The aim of this study was to use liposomal structure consisting prodigiosin and plasmid encoding serial GCA nucleotides (LP/pSGCAN) to reduce inflammation in microglial cells (MGCs) and astrocyte cells (ACCs) by ATM/ATR signaling. Here, it was shown that LP/pSGCAN decreased cell viability and total RNA level. Importantly, LP/pSGCAN had more effect on ACCs than MGCs (P < 0.05). Moreover, increase of apoptosis was seen with increase of concentration. The expression of IL-1 and IL-6 were decreased and the expression of ATM and ATR were increased in treated MGCs and ACCs, which showed LP/pSGCAN could inhibit inflammation by activation of ATM/ATR pathway.

Topics: Animals; Astrocytes; Ataxia Telangiectasia Mutated Proteins; Cells, Cultured; Humans; Inflammation; Liposomes; Microglia; Nucleic Acid Synthesis Inhibitors; Nucleotides; Prodigiosin; Signal Transduction

Topics: Adult; Bronchitis; Child; Cholecystitis; Colitis; Humans; Infant; Infections; Inflammation; Otitis; Periodontitis; Phagocytosis; Prodigiosin; Tonsillitis