preproenkephalin and Chromosome-Deletion

preproenkephalin has been researched along with Chromosome-Deletion* in 1 studies

Other Studies

1 other study(ies) available for preproenkephalin and Chromosome-Deletion

Article	Year
Expression of a human proenkephalin A cDNA in Escherichia coli. Bioscience reports, 1990, Volume: 10, Issue:5 A 1000 base pair cDNA coding for the entire human proenkephalin A(proA) polypeptide was subcloned into the multifunctional pMPV 2911/M E. coli vector. The recombinant plasmid was found to express an approximately 30 kDa prohormone, which was recognized by a Met-Arg6-Phe2 antibody, directed against the C-terminal part of the enkephalin A prohormone. The expression of human proenkephalin A cDNA should thus permit the rapid purification of unfused recombinant enkephalin A prohormone, which itself may provide a model substrate to identify endoproteolytic processing activities. Topics: Chromosome Deletion; Cloning, Molecular; DNA Transposable Elements; DNA, Recombinant; Enkephalins; Escherichia coli; Gene Expression Regulation, Bacterial; Genetic Vectors; Open Reading Frames; Plasmids; Protein Precursors; Recombinant Proteins	1990

Article

Year

Expression of a human proenkephalin A cDNA in Escherichia coli.

Bioscience reports, 1990, Volume: 10, Issue:5

A 1000 base pair cDNA coding for the entire human proenkephalin A(proA) polypeptide was subcloned into the multifunctional pMPV 2911/M E. coli vector. The recombinant plasmid was found to express an approximately 30 kDa prohormone, which was recognized by a Met-Arg6-Phe2 antibody, directed against the C-terminal part of the enkephalin A prohormone. The expression of human proenkephalin A cDNA should thus permit the rapid purification of unfused recombinant enkephalin A prohormone, which itself may provide a model substrate to identify endoproteolytic processing activities.

Topics: Chromosome Deletion; Cloning, Molecular; DNA Transposable Elements; DNA, Recombinant; Enkephalins; Escherichia coli; Gene Expression Regulation, Bacterial; Genetic Vectors; Open Reading Frames; Plasmids; Protein Precursors; Recombinant Proteins

1990