preproenkephalin has been researched along with Brain-Ischemia* in 2 studies
2 other study(ies) available for preproenkephalin and Brain-Ischemia
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Down-regulation of striatal enkephalinergic (PPA) messenger RNA without prior apoptotic features following reversible focal ischemia in rat.
In order to determine whether striatal enkephalinergic neurons were affected by reversible focal ischemia, we have investigated the expression of the preproenkephalin (PPA) messenger by in situ hybridization (ISH) combined with TUNEL staining to display apoptosis in the same rat brain sections. Our data demonstrated a massive reduction of the number of PPA-mRNA containing neurons concomitant with the emergence of apoptotic cells. However, double-labeled neurons (ISH- and TUNEL-positive cells) were not detected, suggesting that either disruption of mRNA precedes DNA fragmentation or ischemia leads to a long lasting reduction of mRNA(s) without damage. Topics: Animals; Apoptosis; Arterial Occlusive Diseases; Biotin; Brain Ischemia; Deoxyuracil Nucleotides; DNA Fragmentation; Enkephalins; In Situ Hybridization; Male; Neostriatum; Protein Precursors; Rats; Rats, Sprague-Dawley; RNA, Messenger; Staining and Labeling | 1997 |
Acute and persistent suppression of preproenkephalin mRNA expression in the striatum following developmental hypoxic-ischemic injury.
The striatum is vulnerable to hypoxic-ischemic injury during development. In a rodent model of perinatal hypoxia-ischemia, it has been shown that striatal neurons are not uniformly vulnerable. Cholinergic neurons and NADPH-diaphorase-positive neurons are relatively spared. However, it is unknown what classes of striatal neurons are relatively sensitive. One of the major classes of striatal neurons uses enkephalin as a neurotransmitter. We have studied the effect of early hypoxic-ischemic injury on this class of neurons using a quantitative solution hybridization assay for preproenkephalin mRNA in conjunction with in situ hybridization. Hypoxia-ischemia results in an early (up to 24 h) decrease in striatal preproenkephalin mRNA, which is shown by in situ hybridization to occur mainly in the dorsal portion of the striatum. By 14 days, whole striatal preproenkephalin mRNA and total enkephalin-containing peptide levels are normal. However, at 14 days, in situ hybridization reveals that regions of complete preproenkephalin mRNA-positive neuron loss remain in the dorsal region. Normal whole striatal levels are due to an up-regulation of preproenkephalin mRNA expression in the ventrolateral region of the injured striatum. Given the important role that the enkephalin-containing striatal efferent projection plays in regulating motor function, its relative loss may be important in the chronic disturbances of motor control observed in brain injury due to developmental hypoxic-ischemic injury. Topics: Aging; Animals; Autoradiography; Brain Ischemia; Corpus Striatum; Enkephalins; Female; Hypoxia, Brain; In Situ Hybridization; NADPH Dehydrogenase; Neurons; Phosphorus Radioisotopes; Protein Precursors; Rats; Rats, Sprague-Dawley; RNA, Messenger; Suppression, Genetic | 1994 |