pregabalin and Pancreatitis

Topics: Analgesics; Animals; Anti-Inflammatory Agents; Benzothiazoles; Binding Sites; Ganglia, Spinal; HEK293 Cells; Humans; Male; Mice, Inbred BALB C; Mice, Inbred C57BL; Molecular Docking Simulation; Molecular Structure; Neurogenic Inflammation; Pain; Pancreatitis; Potassium Channels, Tandem Pore Domain; Protein Binding; Rats, Sprague-Dawley; Structure-Activity Relationship

The aim of this study was to examine pancreatic pathology and the prophylactic effects of pregabalin in lipopolysaccharide (LPS) induced sepsis model in aged rats.. Twenty-four female, one-year-old, Wistar Albino rats were assigned to three groups; Group I (control), Group II (study group: 5mg/kg LPS intraperitoneal, single dose) and Group III(treatment group: 5mg/kg LPS+30 mg/kg oral pregabalin one hour before LPS). Animals were sacrificed by exsanguination 6 hours after LPS administration. Blood and pancreatic tissue samples were collected for biochemical, pathological, and immunohistochemical analyses.. LPS caused increases in serum amylase and lipase level but led to a reduction in glucose levels. Following histopathological analysis, numerous neutrophil leucocyte infiltrations were observed in vessels and pancreatic tissues. Increased caspase-3 expression was observed in both the endocrine and exocrine pancreas in the LPS group. Similarly, IL-6, caspase-3 (Cas-3), inducible nitric oxide synthase (iNOS), granulocyte colony-stimulating factor (G-CSF) and serum amyloid-A (SAA) expressions were increased by LPS. Pregabalin improved biochemical, histopathological, and immunohistochemical findings.. This study showed that LPS causes pathological findings in the pancreas, but pregabalin has ameliorative effects in aged rats with sepsis. Cas-3, IL-6, iNOS, G-CSF, and SAA all play pivotal roles in the pathogenesis of LPS-induced pancreatic damage.

Topics: Animals; Anti-Inflammatory Agents; Caspase 3; Cytoprotection; Disease Models, Animal; Female; Granulocyte Colony-Stimulating Factor; Inflammation Mediators; Interleukin-6; Lipopolysaccharides; Nitric Oxide Synthase Type II; Pancreas; Pancreatitis; Pregabalin; Rats, Wistar; Serum Amyloid A Protein; Signal Transduction