povidone-iodine and Escherichia-coli-Infections

To determine whether antisepsis with povidone-iodine solution applied at the external urethral meatus confers protection against infective complications following transurethral resection of the prostate (TURP) and compare that with antibacterial prophylaxis.. A total of 167 patients with sterile urine undergoing TURP for benign prostatic hyperplasia (BPH) were prospectively randomized into three groups. Group A, had gauze soaked in saline applied at the urethral meatus (control group). In group B, the gauze was soaked in povidone-iodine instead of saline and group C had a single 1 g i.v. injection of cephradine at induction of anaesthesia with no treatment for the meatus. Bacteraemia, post-operative bacteriuria and other infective complications were compared in the three groups. Associations of bacteriuria at catheter removal and intra-operative bacteraemia with infective complications and with long term bacteriuria were also studied.. Bacteriuria rate at catheter removal was not significantly different in the three groups. However, intraoperative bacteraemia and bacterial growth at the external urethral meatus was significantly lower in group C. Bacteriuria at catheter removal was significantly associated with bacterial growth at the meatus but not with long term bacteriuria at 3 months. Bacteriuria at catheter removal could not accurately predict infective complications.. Post-TURP bacteriuria appears to be preceded by bacterial growth at the external urethral meatus. Antisepsis with povidone-iodine solution application at the meatus does not confer adequate protection against meatal bacterial growth as that obtained by prophylactic antibacterials. Nonetheless, neither antibacterial prophylaxis nor local antisepsis could reduce bacteriuria rate in this study.

Topics: Administration, Topical; Aged; Anti-Bacterial Agents; Anti-Infective Agents, Local; Antibiotic Prophylaxis; Bacteriuria; Cephradine; Escherichia coli Infections; Humans; Male; Povidone-Iodine; Prospective Studies; Surgical Wound Infection; Transurethral Resection of Prostate; Urinary Catheterization

We randomized 64 patients with a history of recurrent urinary tract infections among 3 regimens of long-term (1 year) prophylactic treatment: 20 were given 100 mg. trimethoprim at night, 25 received 1,000 mg. methenamine hippurate every 12 hours and 19 were asked to cleanse the perineum (especially the periurethral area) twice daily with povidone-iodine solution. The progress of patients in terms of urinary symptoms and/or bacteriuria, changes in periurethral flora, side effects, and hematological and biochemical profiles was followed at regular intervals. All treatments were effective in reducing the incidence of symptomatic attacks when compared to the 12 months immediately before therapy and there was little to choose between the individual regimens on this account. However, trimethoprim was tolerated better than were the other 2 treatment regimens. In the group given trimethoprim most of the breakthrough infections (71.4 per cent) that occurred were caused by trimethoprim-resistant organisms (usually Escherichia coli), while in the other 2 groups the incidence of trimethoprim-resistant organisms causing infection was low (2.7 per cent). Treatment with trimethoprim reduced significantly the periurethral colonization of Escherichia coli.

Topics: Administration, Topical; Adult; Clinical Trials as Topic; Drug Eruptions; Drug Resistance, Microbial; Escherichia coli Infections; Female; Gastrointestinal Diseases; Hippurates; Humans; Methenamine; Perineum; Povidone; Povidone-Iodine; Random Allocation; Time Factors; Trimethoprim; Urinary Tract Infections

This study aimed to assess the in vitro antimicrobial effects of chlorhexidine (CHX) and povidone-iodine (PI) on clinical isolates of Escherichia coli (E. coli) and Trueperella pyogenes (T. pyogenes) from the vaginal discharge of dairy cows, as well as to compare the cytotoxicity effects of CHX and PI on bovine endometrial epithelial cells (BEnEpC). In Experiment 1, 12 E. coli and 10 T. pyogenes were isolated from the vaginal discharge of cows with a uterine infection. The MIC and MBC against CHX and PI were analyzed in vitro. In Experiment 2, the cytotoxicity effects of CHX and PI on BEnEpC were analyzed using a Viability/Cytotoxicity Kit, wound scratch healing assay, and the expression of pro-inflammatory cytokine genes (IL-6, IL-8, and TNF-α). In Experiment 1, the MIC and MBC values of CHX against E. coli were 0.0002% and 0.0002 to 0.00025%, respectively. The MIC and MBC values of PI were 1.25 to 2.5% and 1.25 to 5%, respectively. For T. pyogenes, the MIC and MBC values of CHX were 0.00002%. The MIC and MBC values of PI were 1.25%. In Experiment 2, the cell viability significantly decreased, and wound closures were significantly inhibited after treatment with ≥ 0.002% CHX and ≥ 0.025% PI. The expression of IL-6, IL-8, and TNF-α significantly increased after treatment with PI. Only IL-6 showed a significant increase after cells were treated with 0.00002% and 0.0002% CHX. The results suggested that both CHX and PI had high antibacterial effects. However, veterinarians and farmers should be aware of their cytotoxicity, which decrease viability of endometrial epithelial cells and inhibit wound healing in vitro.

Topics: Actinomycetaceae; Animals; Anti-Bacterial Agents; Cattle; Cattle Diseases; Chlorhexidine; Endometritis; Escherichia coli; Escherichia coli Infections; Female; Interleukin-6; Interleukin-8; Povidone-Iodine; Tumor Necrosis Factor-alpha; Vaginal Discharge

The gram-negative bacteria secreted endotoxin, Lipopolysaccharide (LPS), plays important roles in the formation and recurrence of hepatolithiasis and chronic biliary inflammation in patients of Southeast Asia. We aimed to elucidate the anti-inflammatory effect and mechanism of local antibiotics irrigation on chronic proliferative cholangitis (CPC) and hepatolithiasis.. Escherichia coli was injected into rabbit bile ducts to induce CPC. Rabbits were divided into sham operation (SO), povidone-iodine, Metronidazole plus chlorhexidine, ofloxacin, furacillin, Neosporin® G.U., and CPC groups. Local irrigation was performed for 28 days after CPC was established. Residual E. coli and LPS, and the expression of MCP-1, CD14, COX-2, VEGF, IL-6, NF-κB, TNF-α, Fas, TGF-β1, α-SMA, Collagen-I, β-glucuronidase, PKC, C-myc, and Mucin 5AC were assessed in bile duct tissues.. The residual E. coli and LPS, and expression of MCP-1, CD14, COX-2, IL-6, NF-κB, TNF-α, Fas, TGF-β1, α-SMA, β-glucuronidase, PKC, C-myc, and Mucin 5AC in the SO, povidone-iodine, Metronidazole plus chlorhexidine, ofloxacin, and Neosporin® G.U. groups were significantly lower than those in the furacillin and CPC groups (P<0.05). VEGF and Collagen-I levels in the SO, povidone-iodine, metronidazole plus chlorhexidine, and ofloxacin groups were significantly lower than those in the furacillin, Neosporin® G.U., and CPC groups (P<0.05).. LPS affects the pathophysiology of E. coli caused chronic proliferative cholangitis and hepatolithiasis recurrence. Local antibiotics irrigation could prevent chronic proliferative cholangitis and stones formation by decreasing LPS-induced proinflammatory and profibrotic cytokines release. Povidone iodine, metronidazole plus chlorhexidine, and ofloxacin were more effective than Neosporin® G.U. and furacillin.

Topics: Animals; Anti-Bacterial Agents; Bacitracin; Chlorhexidine; Cholangitis; Chronic Disease; Collagen Type I; Cytokines; Drug Combinations; Escherichia coli; Escherichia coli Infections; Lipopolysaccharides; Lithiasis; Liver Diseases; Metronidazole; Neomycin; Nitrofurazone; Ofloxacin; Polymyxin B; Povidone-Iodine; Rabbits; Therapeutic Irrigation; Vascular Endothelial Growth Factor A

Lots of microorganisms exist in layer houses can cause bird diseases and worker health concerns. Spraying chemical disinfectants is an effective way to decontaminate pathogenic microorganisms in the air and on surfaces in poultry houses. Slightly acidic electrolyzed water (SAEW, pH 5.0-6.5) is an ideal, environmentally friendly broad-spectrum disinfectant to prevent and control bacterial or viral infection in layer farms. The purpose of this work was to investigate the cleaning effectiveness of SAEW for inactivating the microbes in layer houses. The effect of SAEW was evaluated by solid materials and surface disinfection in a hen house. Results indicate that SAEW with an available chlorine concentration of 250 mg/L, pH value of 6.19, and oxygen reduction potential of 974 mV inactivated 100% of bacteria and fungi in solid materials (dusts, feces, feather, and feed), which is more efficient than common chemical disinfectant such as benzalkonium chloride solution (1:1,000 vol/vol) and povidone-iodine solution (1:1,000 vol/vol). Also, it significantly reduced the microbes on the equipment or facility surfaces (P < 0.05), including floor, wall, feed trough, and water pipe surfaces. Moreover, SAEW effectively decreased the survival rates of Salmonella and Escherichia coli by 21 and 16 percentage points. In addition, spraying the target with tap water before disinfection plays an important role in spray disinfection.

Topics: Animal Husbandry; Animals; Benzalkonium Compounds; Chickens; Disinfectants; Disinfection; Electrolysis; Escherichia coli; Escherichia coli Infections; Female; Housing, Animal; Poultry Diseases; Povidone-Iodine; Salmonella; Salmonella Infections, Animal; Water

To evaluate the effectiveness of the use of the povidone-iodine (PVI) added to the liquid of wash of the peritoneal cavity in the reduction of bacterial absorption and in the remainder non-phagocyted bacteria in the circulating blood of rat.. Thirty four Wistar females rats were used, distributed in the following groups: A (n=10), non-treated; B (n=9), wash of the peritoneal cavity with solution of PVI to 1% in saline solution; C (n=15), wash of the cavity with saline solution. After anesthesia, it was made intraperitoneal infusion of solution of Escherichia coli labeled with 99mTc containing 10(8) CFU/ml. After 40 minutes, it was made the treatment, in the group A, manipulation of the viscera; in the group B, irrigation of the peritoneal cavity with warm solution of 1% PVPI to 37,5 degrees C, and in the group C irrigation with warm saline (37,5 degrees C). After 15 minutes of the treatment, blood samples and fragments of liver, spleen and lung was obtained for count of the radioactivity, and animals killed by abdominal aorta section. There were determined the bacterial absorption index and the remainder index in the bloodstream.. Of the total of bacteria infused in the peritoneum, there was absorption of 0,92% (0,14% to 2,13%) in the animals of the group A (controls), 0,49% (0,18% to 0,71%) after use of topical PVPI (group B) and 0,80% (0,04% to 3,8%) after wash with saline solution (group C). There was significant reduction of the absorption when compared the treated animals with PVPI and the controls (p=0,003). Of the total of bacteria absorbed for the circulatory current, the percentile amount of bacteria non-phagocyted in the outlying blood was of 2,9% (1,1% to 17,7%) in the control group, 15,2% (8,3% to 21,4%) in those treated with PVPI (group B) and 6,9% (0,8% to 29,7%) after wash with saline solution (group C), with difference among controls and treated with PVPI (p=0,01).. The wash of the cavity peritoneal of mice with solution containing PVPI showed to be capable to reduce the absorption of bacteria by peritoneum of rat; however it seems to interfere with the function of the phagocytic cells for the observation of the increase of viable bacteria in the outlying blood of those animals.

Topics: Animals; Anti-Infective Agents, Local; Disease Models, Animal; Drug Evaluation, Preclinical; Escherichia coli Infections; Female; Macrophages, Peritoneal; Peritoneal Cavity; Peritoneal Lavage; Peritonitis; Phagocytosis; Povidone-Iodine; Rats; Rats, Wistar; Solutions

Topics: Anti-Infective Agents, Local; Chlorhexidine; Escherichia coli; Escherichia coli Infections; Povidone-Iodine; Staphylococcal Infections; Staphylococcus; Surgical Wound Infection; Sutures

The risk of infection and potential microbial transmission with EUS-guided FNA (EUS-FNA) of cystic lesions remains unknown.. We developed an in vitro model to study the incidence of transmucosal microbial transmission during EUS-FNA of cystic lesions and to evaluate the in vitro efficacy of bacteriocidal agent washings of mucosa before FNA under experimental conditions.. Conical tubes, 15 mL, filled with aerobic blood culture bottle media were prepared. Then sterile sections of bovine tripe were fastened over the top of the conical tubes in a sterile fashion (conical tube-tripe unit). FNA was performed with 22-gauge FNA needles. A series of 6 experiments were performed. Ten conical tube-tripe units underwent FNA once through the tripe into the blood culture media to ensure sterility. The surface of 10 conical tube-tripe units were inoculated with 50 microL of a 1.5 x 10(8) 1:1 mixture of Escherichia coli (E coli) and Enterococcus sp, and FNA was performed one time into the blood culture media to ensure contamination (controls). The surface of 40 conical tube-tripe units were inoculated with 50 microL of a 1.5 x 10(8) 1:1 mixture of E coli and Enterococcus sp Each of 4 sets of 10 conical tube-tripe units underwent experimental scenarios that consisted of washings with either 1 mL of 0.5% povidone iodine, chlorhexidine, absolute ethanol, or sterile water. FNA was performed once through the tripe into the blood culture media after washing the surface of the tripe. After each conical tube-tripe unit underwent FNA one time, 1 mL blood culture media was obtained and mixed on pour plate agar media and was incubated along with the conical tubes. Microbial evaluation of the conical tubes that contained the blood culture media and pour plates was performed after 48 hours of incubation.. Gastroenterology and Microbiology Departments of Scott White Memorial Hospital and Clinic in Temple, Texas.. EUS-FNA of cystic lesions.. Microbial contamination during EUS-FNA of an in vitro cystic environment.. A control without E coli and Enterococcus sp was with 0% contamination. A control group with E coli and Enterococcus sp was with 100% contamination; sterile water washings, 100% contamination (P = 1.00); iodine washings, 20% contamination (P < .001); chlorhexidine washings, 80% contamination (P = .47); and absolute ethanol washings, 90% contamination (P = 1.00). Results were compared with our control group by statistical tests of proportions by using the Fisher exact test.. EUS-FNA of sterile cystic lesions resulted in transmucosal microbial contamination. However, our model demonstrated that iodine sterilization of a contaminated mucosal surface produced a very highly statistically significant (P < .001) reduction in the transmission of infectious agents into a sterile environment. This in vitro model could translate into clinical practice by providing evidence that microbial transmission by FNA occurred. The utility of povidone iodine washings could alter procedure methods and patient care.

Topics: Animals; Anti-Bacterial Agents; Biopsy, Fine-Needle; Cattle; Cysts; Drug Contamination; Endosonography; Enterococcus; Equipment Contamination; Escherichia coli; Escherichia coli Infections; Gastric Mucosa; Gastrointestinal Diseases; Gram-Positive Bacterial Infections; Humans; Intestinal Mucosa; Pancreatic Cyst; Povidone-Iodine; Sterilization

In pressure sore wounds after spinal cord injury, methicillin-resistant Staphylococcus aureus can be detected in 2% of the cases. The elimination of the germ is the aim of the treatment. Pressure sore wounds are an often found complication after spinal cord injury. For local treatment five commercially available antiseptics for the skin and mucous membrane were tested in vitro. The method used is a modified qualitative and quantitative suspension test. The antiseptics were tested without and with addition of 5% albumin in order to simulate the conditions of the wound in vivo. The results show a superior efficacy of the povidone-iodine preparations. Betadine, probably due to the higher concentration, is more efficacious than Braunol; chlorhexidine is sufficiently efficacious without the addition of albumin. These results still have to be confirmed by in vivo studies.

Topics: Anti-Infective Agents, Local; Biguanides; Chamomile; Chlorhexidine; Escherichia coli; Escherichia coli Infections; Flavonoids; Humans; Imines; Iodophors; Methicillin Resistance; Oils, Volatile; Plants, Medicinal; Povidone-Iodine; Pressure Ulcer; Pseudomonas aeruginosa; Pseudomonas Infections; Pyridines; Quaternary Ammonium Compounds; Serum Albumin; Skin; Spinal Cord Injuries; Staphylococcal Infections; Staphylococcus aureus

We compared a newly developed closed urinary drainage system incorporating a retrograde contamination guard (RCG) with the conventional closed drainage system. The new system contains a solid bactericide (povidone iodine) pellet enclosed in a porous cartridge at the drain port of the urine collection bag. A catheterised rabbit model was used. The urine drainage bags were challenged daily for 8 days with an auxotrophically marked uropathogenic strain of Escherichia coli at the outlet tube. The bag urine was infected with the marked organism in 10 of the 11 rabbits in the control group (conventional collection bags) in 3.8 +/- 1.03 days and in only 1 of the 13 rabbits fitted with the RCG. The RCG effectively prevents contamination of the urine drainage bag and thus may play an important role in reducing the incidence of nosocomial urinary tract infections.

Topics: Animals; Bacteriuria; Equipment Contamination; Escherichia coli Infections; Povidone-Iodine; Rabbits; Urinary Catheterization

Intestinal macromolecular permeability to macromolecules was determined in a group of specific pathogen free piglets before and after they were infected experimentally with Escherichia coli. Six hours after the infection all piglets developed a profuse diarrhoea. The urea and total protein concentrations in the serum increased markedly after the onset of diarrhoea. Haemoglobin concentration and PCV decreased steadily during the experiment but blood glucose concentration and lipid composition of the faeces did not change. No structural abnormalities in the jejunal and ileal mucosa were seen. No uptake of macromolecules (40,000 KDa) was found suggesting that molecules with a molecular weight of 40,000 or more do not play a role in the persistent diarrhoea sometimes seen in piglets after colibacillosis.

Topics: Animals; Animals, Newborn; Diarrhea; Escherichia coli Infections; Ileum; Intestinal Mucosa; Iodine Radioisotopes; Jejunum; Permeability; Povidone-Iodine; Specific Pathogen-Free Organisms; Swine; Swine Diseases

Intraperitoneal lavage with povidone-iodine solution has been reported by some to be beneficial in the treatment of peritonitis and by others to cause local and toxic side effects. In this study, 200 white mice, divided into four groups of 50, were subjected to bacterial peritonitis. The first group had no treatment; peritoneal lavage was carried out using povidone-iodine solution in the second group and a 0.9% sodium chloride solution in the third. In the fourth group, antibiotics (clindamycin and gentamicin) were instilled intraperitoneally without peritoneal lavage. The povidone-iodine solution had no beneficial effect, the death rate after 1 week (76%) being similar to that in the control group (78%) and much higher than that in mice treated with sodium chloride lavage (38%) and antibiotics without lavage (16%). A second series of experiments was, therefore, carried out to investigate the toxic effect of povidone-iodine solution intraperitoneally on mice without peritonitis; the solution was found to be toxic.

Topics: Animals; Escherichia coli Infections; Female; Male; Mice; Peritoneal Lavage; Peritoneum; Peritonitis; Povidone; Povidone-Iodine

A model of bacterial peritonitis, using mice infected with Escherichia coli, has been used to assess the protective effects of intraperitoneal treatment with antiseptics. Of the five antiseptics tested, only chlorhexidine gluconate had any protective effect, concentrations of 0.05 and 0.02 per cent reducing the mortality to 14 and 50 per cent respectively. The other antiseptics, taurolin, noxytiolin, povidone iodine and hypochlorite were all ineffective. Delayed treatment with chlorhexidine was not as effective as instillation immediately postinfection.

Topics: Animals; Anti-Infective Agents, Local; Chlorhexidine; Disease Models, Animal; Escherichia coli Infections; Hypochlorous Acid; Injections, Intraperitoneal; Male; Mice; Noxythiolin; Peritonitis; Povidone-Iodine; Taurine; Thiadiazines; Time Factors

Topics: Administration, Topical; Animals; Anti-Bacterial Agents; Anti-Infective Agents, Local; Cephaloridine; Drug Therapy, Combination; Escherichia coli Infections; Guinea Pigs; Injections, Intramuscular; Male; Postoperative Care; Povidone-Iodine; Surgical Wound Infection

Topics: Animals; Escherichia coli Infections; Hemoglobins; Male; Peritonitis; Povidone; Povidone-Iodine; Rats; Therapeutic Irrigation

In dogs with appendicitis-peritonitis, intraperitoneal povidone-iodine caused death more rapidly than the instillation of saline solution. The bacterial content of canine peritoneal fluid increased with time. Although fewer bacteria were found in fluid from povidone-iodine-treated dogs, the differences were not statistically significant. Qualitative chemical analysis of peritoneal fluid revealed iodide, but not free iodine, 15 to 30 minutes after instillation of povidone-iodine. Iodine was present in the peritoneum at 2 hours but not at 3 or 6 hours. The antibacterial effect of povidone-iodine was demonstrated in mice challenged intraperitoneally with lethal doses of Escherichia coli. Povidone-iodine diminished mortality when injected immediately (p less than 0.005) but not when given 1 to 3 hours later. Immediate injection of povidone-iodine into mice lowered the number of E. coli by 3 logs. Injection of povidone-iodine 3 hours after bacterial challenge lowered the number of E. coli by only 1/3 log. This lesser bactericidal effect in mice is attributed to greater dispersal and sequestration of bacteria throughout the peritoneal cavity with time and with inactivation of povidone-iodine by reduction to iodide in vivo. In dogs with appendicitis-peritonitis, the more rapid death after treatment with povidone-iodine was not associated with differences in peritoneal microflora but with peritoneal absorption of excessive amounts of iodide.

Topics: Animals; Appendicitis; Ascitic Fluid; Bacteroides; Clostridium; Dogs; Escherichia coli; Escherichia coli Infections; Female; Injections, Intraperitoneal; Iodides; Male; Mice; Peritonitis; Povidone; Povidone-Iodine

Eight horses ranging in age from 4 days to 9 years were treated for peritonitis. Escherichia coli was isolated in four cases and Nocardia sp in one case. In each case, a catheter placed in the peritoneal cavity allowed drainage of a large amount of purulent fluid. Retrograde peritoneal lavage was performed through a Foley catheter or medical tubing, using Ringer's lactate solution containing kanamycin, povidone iodine, or nitrofurazone. All except two horses responded well to repeated lavage.

Topics: Animals; Escherichia coli Infections; Female; Horse Diseases; Horses; Male; Nitrofurazone; Peritoneum; Peritonitis; Povidone-Iodine; Therapeutic Irrigation

Topics: Animals; Dose-Response Relationship, Drug; Escherichia coli Infections; Female; Injections, Intraperitoneal; Lethal Dose 50; Mice; Mice, Inbred Strains; Peritonitis; Povidone; Povidone-Iodine; Rats

Topics: Animals; Escherichia coli Infections; Humans; Injections, Intraperitoneal; Mice; Peritonitis; Povidone; Povidone-Iodine; Rats

Topics: Absorption; Animals; Cephalothin; Disease Models, Animal; Escherichia coli Infections; Injections, Intraperitoneal; Iodine; Kanamycin; Peritonitis; Povidone; Povidone-Iodine; Rats; Thyroid Function Tests; Tissue Adhesions

Topics: Bacteria; Burns; Cross Infection; Escherichia coli; Escherichia coli Infections; Evaluation Studies as Topic; Gastroenteritis; Hand; Hexachlorophene; Humans; Hygiene; Infant Care; Intensive Care Units; Medical Staff, Hospital; Methods; Nurseries, Hospital; Nursing Staff, Hospital; Povidone-Iodine; Pseudomonas aeruginosa; Skin; Soaps; Staphylococcus; Streptococcus

Topics: Aged; Bacterial Infections; Cardiac Surgical Procedures; Child, Preschool; Enterobacter; Enterobacteriaceae Infections; Escherichia coli Infections; Female; Humans; Infant; Klebsiella Infections; Male; Mediastinal Diseases; Middle Aged; Povidone; Povidone-Iodine; Proteus Infections; Pseudomonas Infections; Serratia; Staphylococcal Infections; Sternum; Streptococcal Infections; Surgical Wound Infection; Therapeutic Irrigation

Topics: Administration, Topical; Adolescent; Adult; Aged; Burns; Candidiasis; Child; Child, Preschool; Escherichia coli Infections; Female; Humans; Infant; Infection Control; Klebsiella Infections; Male; Middle Aged; Ointments; Povidone; Povidone-Iodine; Proteus Infections; Pseudomonas Infections; Staphylococcal Infections; Streptococcal Infections