polident and Dental-Plaque

To compare ultrasonic cleaning combined with immersion in a commercially available peroxide-based cleanser solution (Polident(®) ) with other denture cleaning methods, we examined the quantity of micro-organisms that survived on dentures before and after various cleaning methods.. One hundred complete dentures belonging to 50 nursing home residents (mean age, 84.6 years) were randomly assigned to five groups according to the cleaning method employed: (A) immersion in Polident(®) solution alone, (B) brushing with water, (C) ultrasonic cleaning with water, (D) method (A) followed by method (B) and (E) ultrasonic cleaning combined with immersion in Polident(®) solution. Before and after the dentures had been cleaned, denture biofilm was collected from the mucosal surface of each lateral half of the examined dentures. The collected micro-organisms were cultured, presumptively identified by standard methods and quantified. Comparisons between the five cleaning methods were carried out using the Kruskal-Wallis test and Dunn's multiple comparisons test.. The denture cleaning methods involving the use of Polident(®) solution (methods A, D and E) were significantly more effective at denture disinfection than the other methods (p < 0.05); in particular, the quantity of Candida spp. was lowest after method E (median, 0.00; significantly lower than those observed after methods A, B and C; p < 0.05).. It was concluded that ultrasonic cleaning combined with immersion in a peroxide-based cleanser solution effectively reduces the quantity of micro-organisms surviving on dentures and is a suitable method for elderly individuals who find brushing their dentures difficult.

Topics: Aged; Aged, 80 and over; Biofilms; Borates; Candida; Dental Devices, Home Care; Dental Disinfectants; Dental Plaque; Denture Cleansers; Denture, Complete; Female; Humans; Male; Microbial Viability; Middle Aged; Neisseria; Peroxides; Random Allocation; Sonication; Streptococcus; Sulfates; Ultrasonic Waves; Water

To investigate effective denture-cleaning methods, we examined the relationships between the quantity of microorganisms adhering to dentures and the use of a denture brush and the frequency of use of a denture cleanser.. Denture plaque was collected from the mucosal surface of the examined dentures, which were 142 and 80 upper and lower complete dentures, respectively, worn by 96 outpatients (mean age: 71.9 years) of a university hospital and 41 nursing home residents (mean age: 84.8 years). The collected microorganisms were counted in terms of isolated representative colonies that were cultured and identified using standard methods. The use of a denture brush, the frequency of use, and the type and soaking time of denture cleansers as denture-cleaning methods were surveyed.. The quantity of microorganisms was significantly lower in dentures of denture brush users than in those of non-users in the outpatients (p < 0.01, Mann-Whitney U test). The quantity of microorganisms was significantly lower in the dentures of outpatients who used a denture cleanser daily or 3-4 times a week than in those who used one once or less per month and in the dentures of nursing home residents who used one daily than in those who used one at other frequencies (p < 0.05, Kruskal-Wallis test, followed by Dunn's Multiple Comparison test).. Within the limitations of this study, it was concluded that the use of a denture brush and daily use of denture cleanser should be recommended to complete dentures wearers as denture-cleaning methods that effectively reduce the quantity of microorganisms adhering to dentures.

Topics: Aged; Aged, 80 and over; Bacteria; Bacterial Adhesion; Bacterial Load; Bacteriological Techniques; Borates; Candida; Dental Plaque; Denture Bases; Denture Cleansers; Denture, Complete; Denture, Complete, Lower; Denture, Complete, Upper; Female; Humans; Male; Neisseria; Nursing Homes; Oral Hygiene; Outpatients; Sex Factors; Streptococcus; Sulfates; Time Factors; Toothbrushing

Elevated proportions of Candida albicans in biofilms formed on dentures are associated with stomatitis whereas Streptococcus mutans accumulation on restorative materials can cause secondary caries. Candida albicans, S. mutans, saliva-derived and C. albicans/saliva-derived mixed biofilms were grown on different materials including acrylic denture, porcelain, hydroxyapatite (HA), and polystyrene. The resulting biomass was analysed by three-dimensional image quantification and assessment of colony-forming units. The efficacy of biofilm treatment with a dissolved denture cleansing tablet (Polident(®)) was also evaluated by colony counting. Biofilms formed on HA exhibited the most striking differences in biomass accumulation: biofilms comprising salivary bacteria accrued the highest total biomass whereas C. albicans biofilm formation was greatly reduced on the HA surface compared with other materials, including the acrylic denture surface. These results substantiate clinical findings that acrylic dentures can comprise a reservoir for C. albicans, which renders patients more susceptible to C. albicans infections and stomatitis. Additionally, treatment efficacy of the same type of biofilms varied significantly depending on the surface. Although single-species biofilms formed on polystyrene surfaces exhibited the highest susceptibility to the treatment, the most surviving cells were recovered from HA surfaces for all types of biofilms tested. This study demonstrates that the nature of a surface influences biofilm characteristics including biomass accumulation and susceptibility to antimicrobial treatments. Such treatments should therefore be evaluated on the surfaces colonized by the target pathogen(s).

Topics: Acrylic Resins; Anti-Infective Agents; Bacterial Adhesion; Bacterial Load; Biofilms; Biomass; Borates; Candida albicans; Dental Materials; Dental Plaque; Dental Porcelain; Denture Bases; Denture Cleansers; Dentures; Durapatite; Humans; Imaging, Three-Dimensional; Materials Testing; Microbial Sensitivity Tests; Microscopy, Confocal; Polystyrenes; Saliva; Streptococcus mutans; Sulfates; Surface Properties

Formation of plaque on the surfaces of dentures is a common problem among denture wearers. A study was conducted to determine the distribution of plaque on dentures. The plaque material was disclosed with a dye solution and measured with a modified Quigley-Hein scale. A photographic method was used to determine the distribution of plaque on the dentures of a group of complete-denture wearers. The effectiveness of a perborate soak-type cleanser was also measured by studying the precleaned and postcleaned states of the denture. Denture plaque was more evident on the fitting surfaces of the dentures than on areas of the flange, teeth, and palate. The use of the soak-type cleanser alone may not be completely effective for the control of heavy plaque.

Topics: Aged; Biofilms; Borates; Dental Plaque; Dental Plaque Index; Denture Cleansers; Denture, Complete; Evaluation Studies as Topic; Humans; Middle Aged; Sulfates