pkh-26 and Leukemia--Myeloid--Acute

pkh-26 has been researched along with Leukemia--Myeloid--Acute* in 2 studies

Other Studies

2 other study(ies) available for pkh-26 and Leukemia--Myeloid--Acute

Article	Year
Intercellular adhesion can be visualized using fluorescently labeled fibrosarcoma HT1080 cells cocultured with hematopoietic cell lines or CD34(+) enriched human mobilized peripheral blood cells. Cytometry, 2000, Jun-01, Volume: 40, Issue:2 Intercellular contacts between adjacent cells migrating over each other are important in many cellular processes. However, it has been difficult to visualize and identify dynamic intercellular adhesions between migrating cells in situ.. Two fluorescent membrane dyes, PKH2 and PKH26 for staining HT1080 and hematopoietic cells and cell lines, and an automated fluorescence microscopy system were used to monitor intercellular adhesion.. Cellular extensions connecting two or more adjacent cells were visualized, showing the intercellular adhesion between migrating cells for minutes and up to hours. After cells adhered to each other, followed by cell migration in different directions, cellular extensions were dragged from the pivotal contact points in different focal planes. CD34(+)-enriched mobilized peripheral blood cells and six hematopoietic cell lines showed intercellular connections in cocultures with HT1080. However, the frequency of intercellular connections was variable in different cocultures. A cell density of about 3.1 x 10(4) cells/cm(2) for both cell lines in cocultures provided an adequate number of cells in each field of view, showing up to four intercellular connections per 100 total cells plated.. The tools derived from this study will open new areas of investigation for understanding the mechanism of the intercellular adhesion process. Topics: Antigens, CD34; Burkitt Lymphoma; Cell Adhesion; Cell Count; Cell Culture Techniques; Fibrosarcoma; Fluorescent Dyes; Hematopoietic Stem Cells; Humans; Leukemia, B-Cell; Leukemia, Myeloid, Acute; Leukemia, T-Cell; Microscopy, Fluorescence; Organic Chemicals; Tumor Cells, Cultured	2000
Flow cytometric analysis of natural killer cell function as a clinical assay. Cytometry, 1994, May-01, Volume: 16, Issue:1 The 51Cr release assay has been the method of choice in analyzing natural killer cell (NK) function. Previous FCM cytotoxicity assays of NK activity have had numerous disadvantages that discouraged clinicians from attempting to evaluate NK function by flow cytometry. We demonstrate the effectiveness of using PKH-26, a stable membrane dye, to label the K562 target cells and propidium iodide intercalation into killed target cell DNA to determine the percentage of target cells killed by effector NK cells from the peripheral blood or bone marrow. This method compares favorably with the 51Cr release assay and is quicker and easier to perform. The percentage of cytotoxicity of NK cells (CD3- CD56+ and/or CD16+) from 10 normal subjects and 10 HIV-infected children are reported to demonstrate the feasibility of studying NK function in clinical populations by FCM. The potentiation of cytolysis by alpha-interferon and interleukin 2 in vitro was also compared between these two study groups. In addition, a patient whose leukemic blasts expressed CD56+ was also studied for NK activity using this flow cytometric assay. The benefits of using this flow cytometric approach to clinically assess NK function are discussed. Topics: Adolescent; Adult; Child; Child, Preschool; Chromium Radioisotopes; Cytotoxicity Tests, Immunologic; Flow Cytometry; Fluorescent Dyes; HIV Infections; Humans; Immunophenotyping; Killer Cells, Natural; Leukemia, Myeloid, Acute; Organic Chemicals; Tumor Cells, Cultured	1994

Article

Year

Intercellular adhesion can be visualized using fluorescently labeled fibrosarcoma HT1080 cells cocultured with hematopoietic cell lines or CD34(+) enriched human mobilized peripheral blood cells.

Cytometry, 2000, Jun-01, Volume: 40, Issue:2

Intercellular contacts between adjacent cells migrating over each other are important in many cellular processes. However, it has been difficult to visualize and identify dynamic intercellular adhesions between migrating cells in situ.. Two fluorescent membrane dyes, PKH2 and PKH26 for staining HT1080 and hematopoietic cells and cell lines, and an automated fluorescence microscopy system were used to monitor intercellular adhesion.. Cellular extensions connecting two or more adjacent cells were visualized, showing the intercellular adhesion between migrating cells for minutes and up to hours. After cells adhered to each other, followed by cell migration in different directions, cellular extensions were dragged from the pivotal contact points in different focal planes. CD34(+)-enriched mobilized peripheral blood cells and six hematopoietic cell lines showed intercellular connections in cocultures with HT1080. However, the frequency of intercellular connections was variable in different cocultures. A cell density of about 3.1 x 10(4) cells/cm(2) for both cell lines in cocultures provided an adequate number of cells in each field of view, showing up to four intercellular connections per 100 total cells plated.. The tools derived from this study will open new areas of investigation for understanding the mechanism of the intercellular adhesion process.

Topics: Antigens, CD34; Burkitt Lymphoma; Cell Adhesion; Cell Count; Cell Culture Techniques; Fibrosarcoma; Fluorescent Dyes; Hematopoietic Stem Cells; Humans; Leukemia, B-Cell; Leukemia, Myeloid, Acute; Leukemia, T-Cell; Microscopy, Fluorescence; Organic Chemicals; Tumor Cells, Cultured

2000

Flow cytometric analysis of natural killer cell function as a clinical assay.

Cytometry, 1994, May-01, Volume: 16, Issue:1

The 51Cr release assay has been the method of choice in analyzing natural killer cell (NK) function. Previous FCM cytotoxicity assays of NK activity have had numerous disadvantages that discouraged clinicians from attempting to evaluate NK function by flow cytometry. We demonstrate the effectiveness of using PKH-26, a stable membrane dye, to label the K562 target cells and propidium iodide intercalation into killed target cell DNA to determine the percentage of target cells killed by effector NK cells from the peripheral blood or bone marrow. This method compares favorably with the 51Cr release assay and is quicker and easier to perform. The percentage of cytotoxicity of NK cells (CD3- CD56+ and/or CD16+) from 10 normal subjects and 10 HIV-infected children are reported to demonstrate the feasibility of studying NK function in clinical populations by FCM. The potentiation of cytolysis by alpha-interferon and interleukin 2 in vitro was also compared between these two study groups. In addition, a patient whose leukemic blasts expressed CD56+ was also studied for NK activity using this flow cytometric assay. The benefits of using this flow cytometric approach to clinically assess NK function are discussed.

Topics: Adolescent; Adult; Child; Child, Preschool; Chromium Radioisotopes; Cytotoxicity Tests, Immunologic; Flow Cytometry; Fluorescent Dyes; HIV Infections; Humans; Immunophenotyping; Killer Cells, Natural; Leukemia, Myeloid, Acute; Organic Chemicals; Tumor Cells, Cultured

1994