pkh-26 and Diabetes-Mellitus--Type-1

pkh-26 has been researched along with Diabetes-Mellitus--Type-1* in 1 studies

Other Studies

1 other study(ies) available for pkh-26 and Diabetes-Mellitus--Type-1

Article	Year
Tracking of murine spleen cells in vivo: detection of PKH26-labeled cells in the pancreas of non-obese diabetic (NOD) mice. Journal of immunological methods, 1994, Mar-29, Volume: 170, Issue:1 Flow cytometry was used to track the in vivo migration of PKH26-labeled donor spleen cells from diabetic NOD mice that were injected into non-diabetic recipient NOD mice. Flow cytometric analysis of recipient mouse tissues revealed that the donor cells were present in the peripheral blood, spleen and lymph nodes 24 h following injection and could still be detected after 28 days. PKH26(+) cells were also detectable in the pancreas 7 days after injection. Phenotypic analysis of the PKH26(+) cells that migrated into these target organs and tissues showed that the major cell population detected was Thy1.2(+) T-lymphocytes, predominantly the Thy1.2(+)/L3T4(+) subpopulation, but Thy1.2(+)/Lyt2(+) cells as well as B220(+) cells (B lymphocytes) were also present. Topics: Animals; B-Lymphocytes; Cell Movement; Diabetes Mellitus, Type 1; Female; Flow Cytometry; Fluorescent Dyes; Immunophenotyping; Lymph Nodes; Mice; Mice, Inbred NOD; Organic Chemicals; Pancreas; Spleen; T-Lymphocytes	1994

Article

Year

Tracking of murine spleen cells in vivo: detection of PKH26-labeled cells in the pancreas of non-obese diabetic (NOD) mice.

Journal of immunological methods, 1994, Mar-29, Volume: 170, Issue:1

Flow cytometry was used to track the in vivo migration of PKH26-labeled donor spleen cells from diabetic NOD mice that were injected into non-diabetic recipient NOD mice. Flow cytometric analysis of recipient mouse tissues revealed that the donor cells were present in the peripheral blood, spleen and lymph nodes 24 h following injection and could still be detected after 28 days. PKH26(+) cells were also detectable in the pancreas 7 days after injection. Phenotypic analysis of the PKH26(+) cells that migrated into these target organs and tissues showed that the major cell population detected was Thy1.2(+) T-lymphocytes, predominantly the Thy1.2(+)/L3T4(+) subpopulation, but Thy1.2(+)/Lyt2(+) cells as well as B220(+) cells (B lymphocytes) were also present.

Topics: Animals; B-Lymphocytes; Cell Movement; Diabetes Mellitus, Type 1; Female; Flow Cytometry; Fluorescent Dyes; Immunophenotyping; Lymph Nodes; Mice; Mice, Inbred NOD; Organic Chemicals; Pancreas; Spleen; T-Lymphocytes

1994