pkh-26 has been researched along with Burkitt-Lymphoma* in 2 studies
2 other study(ies) available for pkh-26 and Burkitt-Lymphoma
| Article | Year |
|---|---|
Spontaneous membrane transfer through homotypic synapses between lymphoma cells.
Formation of an immunological synapse by T, B, or NK cells is associated with an intercellular transfer of some membrane fragments from their respective target cells. This capture is thought to require effector cell activation by surface recognition of stimulatory ligand(s). However, spontaneous synaptic transfers between homotypic lymphoid cells has never been described. In this study, we show that without adding Ag, resting healthy lymphoid cells and several tumor cell lines are inactive. Conversely, however, some leukemia cell lines including the Burkitt's lymphoma Daudi continuously uptake patches of autologous cell membranes. This intercellular transfer does not involve cytosol molecules or exosomes, but requires cell contact. In homotypic Daudi cell conjugates, this occurs through immunological synapses, involves constitutive protein kinase C and mitogen-activated protein/extracellular signal-regulated kinase kinase activity and strongly increases upon B cell receptor activation. Thus, spontaneous homosynaptic transfer may reflect the hitherto unsuspected autoreactivity of some leukemia cell lines. Topics: Biological Transport, Active; Burkitt Lymphoma; Cell Communication; Cell Line, Tumor; Cell Membrane; Cytosol; Fluorescent Dyes; Humans; Intercellular Junctions; Lymphoma, B-Cell; Organic Chemicals; Receptors, Antigen, B-Cell; Rhodamines | 2003 |
Intercellular adhesion can be visualized using fluorescently labeled fibrosarcoma HT1080 cells cocultured with hematopoietic cell lines or CD34(+) enriched human mobilized peripheral blood cells.
Intercellular contacts between adjacent cells migrating over each other are important in many cellular processes. However, it has been difficult to visualize and identify dynamic intercellular adhesions between migrating cells in situ.. Two fluorescent membrane dyes, PKH2 and PKH26 for staining HT1080 and hematopoietic cells and cell lines, and an automated fluorescence microscopy system were used to monitor intercellular adhesion.. Cellular extensions connecting two or more adjacent cells were visualized, showing the intercellular adhesion between migrating cells for minutes and up to hours. After cells adhered to each other, followed by cell migration in different directions, cellular extensions were dragged from the pivotal contact points in different focal planes. CD34(+)-enriched mobilized peripheral blood cells and six hematopoietic cell lines showed intercellular connections in cocultures with HT1080. However, the frequency of intercellular connections was variable in different cocultures. A cell density of about 3.1 x 10(4) cells/cm(2) for both cell lines in cocultures provided an adequate number of cells in each field of view, showing up to four intercellular connections per 100 total cells plated.. The tools derived from this study will open new areas of investigation for understanding the mechanism of the intercellular adhesion process. Topics: Antigens, CD34; Burkitt Lymphoma; Cell Adhesion; Cell Count; Cell Culture Techniques; Fibrosarcoma; Fluorescent Dyes; Hematopoietic Stem Cells; Humans; Leukemia, B-Cell; Leukemia, Myeloid, Acute; Leukemia, T-Cell; Microscopy, Fluorescence; Organic Chemicals; Tumor Cells, Cultured | 2000 |