pituitrin and Lymphoma--T-Cell

Topics: Aged, 80 and over; Female; Head and Neck Neoplasms; Humans; Immunohistochemistry; Interleukin-6; Lymphoma, T-Cell; Neoplasm Proteins; Vasopressins

We report three lymphoma patients in whom the syndrome of inappropriate secretion of antidiuretic hormone (SIADH) was observed during the course of lymphoma-associated hemophagocytic syndrome (LAHS). The clinical course was devoid of any known mechanism for SIADH which could be attributable to lymphoma or antineoplastic treatment. Alternatively, high serum levels of interleukin-1 beta and tumor necrosis factor-alpha, which stimulate the secretion of antidiuretic hormone, may have contributed to the development of SIADH in our patients, who were receiving glucocorticoids. In conclusion, LAHS patients should be considered to be at high risk for SIADH.

Topics: Aged; Female; Histiocytosis, Non-Langerhans-Cell; Humans; Inappropriate ADH Syndrome; Interleukin-1; Lymphoma; Lymphoma, T-Cell; Male; Middle Aged; Nose Neoplasms; Risk Factors; Sodium; Tumor Necrosis Factor-alpha; Vasopressins

Studies were carried out to characterize the interaction between inositol 1,4,5-trisphosphate (IP3) receptors and the plasma membrane fraction. Extraction of the membranes with the nonionic detergents Nonidet P-40 and Triton X-100, followed by centrifugation at 100,000 g, resulted in the doubling of the IP3 receptor in the pellets, whereas no detectable binding was found in the supernatants. These data indicate that the detergents did not solubilize the receptor, that it remained associated with membrane particles, and that it is likely to be associated with the cytoskeleton. The cytoskeleton proteins actin, ankyrin, and spectrin were identified in the plasma membrane fraction. However, comparison of the amount of these proteins in different fractions of the detergent, or otherwise treated plasma membrane fractions, showed no direct correlation between the presence of any of these proteins in the plasma membrane fraction and their ability to bind [3H]IP3. This is in contrast to the brain and T-lymphoma cells in which the IP3 receptor is attached to ankyrin (L. Y. W. Bourguigon, H. Jin, N. Iida, N. R. Brandt, and S. H. Zhang. J. Biol. Chem. 268: 6477-6486, 1993; and S. K. Joseph and S. Samanta. J. Biol. Chem 268: 6477-6486, 1993). Thus the hepatic IP3 receptor, which is different from the brain receptor, might attach to the cytoskeleton by anchoring to a different protein. Because cytochalasin D treatment of livers diminishes the ability of IP3 to raise cytosolic free Ca2+ levels, the attachment of the IP3 receptor to the cytoskeleton seems to involve an association with microfilaments.

Topics: Actins; Animals; Ankyrins; Blotting, Western; Brain; Calcium Channels; Cell Membrane; Cytochalasin D; Cytoskeleton; Electrophoresis, Polyacrylamide Gel; Gelsolin; Gluconeogenesis; Inositol 1,4,5-Trisphosphate; Inositol 1,4,5-Trisphosphate Receptors; Kinetics; Liver; Lymphoma, T-Cell; Male; Oxygen Consumption; Phosphorylase a; Rats; Rats, Sprague-Dawley; Receptors, Cytoplasmic and Nuclear; Tetracaine; Vasopressins