pituitrin and Kidney-Neoplasms

Topics: Adolescent; Bronchial Neoplasms; Carcinoma, Small Cell; Child, Preschool; Cushing Syndrome; Diagnosis, Differential; Hormones, Ectopic; Humans; Hyperaldosteronism; Hypercalcemia; Hyperparathyroidism; Hyponatremia; Kidney Neoplasms; Paraneoplastic Endocrine Syndromes; Renin; Sodium Chloride; Syndrome; Vasopressins

Topics: Adrenal Glands; Aldosterone; Feedback; Glomerular Filtration Rate; Humans; Hyponatremia; Kidney; Kidney Diseases; Kidney Neoplasms; Mixed Function Oxygenases; Natriuresis; Sodium; Vasopressins; Water Intoxication

Vasopressin type-2 receptor (V2R) is ectopically expressed and plays a pathogenic role in clear cell renal cell carcinoma (ccRCC) tumor cells. Here we examined how V2R signaling within human ccRCC tumor cells (Caki1 cells) stimulates stromal cancer-associated fibroblasts (CAFs). We found that cell culture conditioned media from Caki1 cells increased activation, migration, and proliferation of fibroblasts in vitro, which was inhibited by V2R gene silencing in Caki1 cells. Analysis of the conditioned media and mRNA of the V2R gene silenced and control Caki1 cells showed that V2R regulates the production of CAF-activating factors. Some of these factors were also found to be regulated by YAP in these Caki1 cells. YAP expression colocalized and correlated with V2R expression in ccRCC tumor tissue. V2R gene silencing or V2R antagonist significantly reduced YAP in Caki1 cells. Moreover, the V2R antagonist reduced YAP expression and myofibroblasts in mouse xenograft tumors. These results suggest that V2R plays an important role in secreting pro-fibrotic factors that stimulate fibroblast activation by a YAP-dependent mechanism in ccRCC tumors. Our results demonstrate a novel role for the V2R-YAP axis in the regulation of myofibroblasts in ccRCC and a potential therapeutic target.

Topics: Animals; Antidiuretic Hormone Receptor Antagonists; Cancer-Associated Fibroblasts; Carcinoma, Renal Cell; Cell Line, Tumor; Culture Media, Conditioned; Fibroblasts; Humans; Kidney; Kidney Neoplasms; Mice; Receptors, Vasopressin; Tumor Microenvironment; Vasopressins; Xenograft Model Antitumor Assays

Responsiveness of cyclic adenosine 3':5'-monophosphate (cAMP) to parathyroid hormone, calcitonin, and vasopressin was studied in six human renal adenocarcinoma cell lines. Four of six renal adenocarcinoma cell lines showed increased cAMP content in response to calcitonin while the other two did not. Neither parathyroid hormone nor vasopressin increased the concentration of cAMP in each of these cell lines. The growth rate of KU-2 cells, which responded to calcitonin with an increase of cAMP content, was inhibited by calcitonin. On the other hand the growth rate of calcitonin-nonsensitive KH-39 cells was unaltered. The growth inhibitory effect of the hormone on KU-2 cells could be considered to be mediated by the increased cAMP levels from the following results: (a) there was positive correlation between the cellular cAMP content and growth inhibition after various amounts of calcitonin addition; (b) KU-2 growth was also suppressed by N6,O2'-dibutyryl cAMP; and (c) a group of KU-2 cells which had become resistant to calcitonin-induced growth inhibition showed a diminished cAMP increase in response to calcitonin.

Topics: Adenocarcinoma; Calcitonin; Cell Line; Cyclic AMP; Humans; Kidney Neoplasms; Parathyroid Hormone; Vasopressins

The arterial blood flow distribution between tumour and intact renal tissue was investigated in rats with transplanted sarcomas. Changes in tissue flow were measured by the microsphere tracer technique, and the redistributing effects on blood flow of vasopressin, noradrenalin and oxytocin was recorded. Bolus injections of vasopressin gave a transient decrease of intact tissue flow, not found in tumours. At increasing doses of vasopressin and in early tumour growth phase, the flow discriminating effect tended to vanish. Constant intravenous infusion of vasopressin gave similar reduction of flow in tumour and intact tissue. Selective administration into the renal artery reduced flow in intact tissue but produced ambiguous effects in tumour tissue. Noradrenalin produced less reduction of tumour flow as compared with intact tissue flow. Oxytocin increased tumour blood flow while no flow change occurred in intact tissue. Oxytocin thus appeared to produce the most favourable redistribution of flow within tumour kidneys for the prospect of conveying cytotoxic agents selectively to the tumour.

Topics: Animals; Female; Kidney; Kidney Neoplasms; Male; Norepinephrine; Oxytocin; Rats; Regional Blood Flow; Sarcoma, Experimental; Vasopressins

The value of the catheter occlusion therapy is analyzed in 92 cases retrospectively, and the indication, and complication risk of these procedures are considered. In the following vessel areas occlusion with different methods have been performed with decreasing frequency; kidneys, pelvis, legs, gastrointestinal tract, external carotid and internal carotid artery. Serious complications have been observed in therapeutic investigations at the head, especially in occlusions of the external carotid artery. In this group embolisation therapy should be performed with strict indication only.

Topics: Adrenal Gland Neoplasms; Cavernous Sinus; Embolization, Therapeutic; Female; Femoral Artery; Gastrointestinal Hemorrhage; Glomus Jugulare Tumor; Humans; Iliac Artery; Kidney Neoplasms; Neoplasm Recurrence, Local; Ovarian Neoplasms; Renal Artery; Vasopressins

Topics: Adenocarcinoma; Adenylyl Cyclases; Animals; Biological Transport; Cell Membrane Permeability; Cyclic AMP; Cyclic GMP; Dogs; Kidney; Kidney Glomerulus; Kidney Medulla; Kidney Neoplasms; Kidney Tubules, Collecting; Kidney Tubules, Distal; Kidney Tubules, Proximal; Loop of Henle; Nephrectomy; Parathyroid Hormone; Prostaglandins; Rabbits; Rats; Vasopressins

Pharmacoangiography with four vasoactive drugs was performed in experimental renal and hepatic tumours in rats in order to compare their ability to demonstrate tumour vascularity. Three vasoconstrictors, angiotensin, norepinephrine and vasopressin, and one vasodilator, tolazoline, were tested, vasoconstrictors giving most diagnostic information and the difference in effect among these sometimes being small and probably dose-related. This diagnostic effect is based upon the primitive character of tumour vessels, being unable to react upon a vasoactive stimulus. Angiotensin turned out to be the superior drug which corresponds to clinical experiences of this drug.

Topics: Angiography; Angiotensin II; Animals; Colonic Neoplasms; Kidney Neoplasms; Liver Neoplasms; Neoplasm Transplantation; Neoplasms, Experimental; Norepinephrine; Rats; Rats, Inbred Strains; Sarcoma, Experimental; Tolazoline; Vasopressins

Topics: Biological Assay; Child, Preschool; Extracellular Space; Female; Humans; Hyponatremia; Kidney Neoplasms; Medication Errors; Osmolar Concentration; Sodium Chloride; Specific Gravity; Urine; Vasopressins; Vincristine; Water-Electrolyte Balance; Wilms Tumor

Ectopic production of polypeptide hormones by tumors of nonendocrine tissues can serve as a clue to diagnosis of the tumor and as a focus for management of the patient with cancer. In the differential diagnosis of syndromes of endocrine hyperfunction, the ectopic hormone syndromes have achieved an increasingly prominent position. Available evidence on the properties of ectopic ACTH, MSH, parathyroid hormone, erythropoietin, gonadotropins, and thyrotropin is consistent with the unifying hypothesis of genetic derepression.

Topics: Abdominal Neoplasms; Adenocarcinoma; Adrenocortical Hyperfunction; Brain Neoplasms; Carcinoma, Bronchogenic; Carcinoma, Hepatocellular; Carcinoma, Small Cell; Cysts; Diagnosis, Differential; Fibroma; Hemangiosarcoma; Humans; Hyperparathyroidism; Hypoglycemia; Kidney Diseases; Kidney Neoplasms; Liver Neoplasms; Lung Neoplasms; Paraneoplastic Endocrine Syndromes; Pheochromocytoma; Polycythemia; Sarcoma; Thoracic Neoplasms; Vasopressins

Topics: Adult; Aged; Blood Circulation Time; Blood Flow Velocity; Bradykinin; Female; Humans; Kidney; Kidney Diseases; Kidney Diseases, Cystic; Kidney Neoplasms; Male; Middle Aged; Radiography; Regional Blood Flow; Renal Artery; Technology, Radiologic; Vasopressins

Topics: Animals; Ascorbic Acid; Biological Assay; Cricetinae; Diethylstilbestrol; Female; In Vitro Techniques; Kidney Neoplasms; Luteinizing Hormone; Male; Organ Size; Ovary; Pituitary Gland; Rats; Vasopressins