pituitrin and Carcinoma

Topics: Adenocarcinoma; Amine Oxidase (Copper-Containing); Antigens, Neoplasm; Calcitonin; Carcinoma; Carcinoma, Small Cell; Carcinoma, Squamous Cell; Creatine Kinase; Humans; Lung Neoplasms; Microscopy, Electron; Neurophysins; Vasopressins

Topics: Adenocarcinoma; Adrenocorticotropic Hormone; Adult; Carcinoma; Carcinoma, Bronchogenic; Carcinoma, Small Cell; Carcinoma, Squamous Cell; Chorionic Gonadotropin; Cushing Syndrome; Dexamethasone; Gynecomastia; Humans; Hypercalcemia; Lomustine; Lung Neoplasms; Male; Mechlorethamine; Neoplasm Metastasis; Neostigmine; Parathyroid Hormone; Smoking; Vasopressins; Water-Electrolyte Balance

Topics: Adenocarcinoma; Adrenocorticotropic Hormone; Arginine; Biliary Tract Diseases; Bronchial Neoplasms; Carcinoma; Chorionic Gonadotropin; Colonic Neoplasms; Cushing Syndrome; Erythropoietin; Female; Follicle Stimulating Hormone; Growth Hormone; Gynecomastia; Hormones, Ectopic; Humans; Hypercalcemia; Lactation Disorders; Lung Neoplasms; Luteinizing Hormone; Models, Biological; Neoplasms; Paraganglioma; Paraneoplastic Endocrine Syndromes; Polycythemia; Pregnancy; Prolactin; Thyroid Neoplasms; Vasopressins

Topics: Adrenal Glands; Adrenocorticotropic Hormone; Animals; Automation; Carcinoma; Corticosterone; Cyclic AMP; Cycloheximide; Fluorescence; Fluorometry; Lung Neoplasms; Membrane Potentials; Methods; Pituitary Gland; Rats; Time Factors; Tissue Extracts; Vasopressins

Intraperitoneal administration of 5-fluorouracil for the treatment of gastrointestinal malignancies results in a greater total drug exposure in the peritoneal fluid than in plasma. Drugs are eliminated from the peritoneal cavity mainly by capillaries leading to the portal venous system and to a lesser extent by lymphatics. The drug itself and the presence of peritoneal carcinomatosis may affect elimination of the drug. The 133Xe-clearance technique allows the influence of a vasoactive agent on the peritoneal blood flow to be estimated with minimal invasiveness. The aim of the present study was to explore whether intraperitoneal 5-FU or peritoneal carcinomatosis affects the peritoneal blood flow and its reactivity to intravenous vasopressin, as measured indirectly with the 133Xe-clearance technique.. The animals used in this study were 63 Wistar-Fu (W-Fu) rats and 67 Lister-Hooded (LH) rats. On day 0, either 5-FU at 25 mg/kg body weight in 25 ml/kg isotonic saline was instilled intraperitoneally, or 1 x 10(5) syngeneic tumour cells were inoculated intraperitoneally. On days 1, 2 and 3 in the 5-FU-treated rats, and on days 12-16 in rats inoculated with tumour cells, peritoneal blood flow was analysed with the 133Xe-clearance technique, before and during intravenous infusion of vasopressin at 0.07 IU/min/kg body weight.. The basal 133Xe-clearance before administration of vasopressin was similar in all groups except in the LH rats treated with 5-FU in which it was significantly lower. Infusion of vasopressin induced a significant decrease in 133Xe-clearance of the same magnitude in controls and in tumour-bearing rats. In the rats given intraperitoneal 5-FU, vasopressin did not reduce the 133Xe-clearance the first day after administration of 5-FU.. Intravenous vasopressin at 0.07 IU/min/kg decreased peritoneal blood flow as measured indirectly with the 133Xe-clearance method. Intraperitoneal 5-FU abrogated the reduction in peritoneal blood flow with intravenous vasopressin the first day after treatment. In contrast, the presence of peritoneal carcinomatosis did not influence peritoneal blood flow, nor the effect of vasopressin

Topics: Animals; Antimetabolites, Antineoplastic; Carcinoma; Fluorouracil; Hemostatics; Infusions, Intravenous; Infusions, Parenteral; Neoplasms, Experimental; Peritoneal Neoplasms; Peritoneum; Rats; Rats, Wistar; Regional Blood Flow; Vasopressins; Xenon Radioisotopes

We studied the putative role of the vasopressin receptors in the phenotypic response of steroid-secreting adrenocortical tumors. A retrospective analysis of a series of 26 adrenocortical tumors responsible for Cushing's syndrome (19 adenomas and 7 carcinomas) showed that vasopressin (10 IU, i.m., lysine vasopressin) induced an ACTH-independent cortisol response (arbitrarily defined as a cortisol rise above baseline of 30 ng/mL or more) in 7 cases (27%). In comparison, 68 of 90 patients with Cushing's disease (76%) had a positive cortisol response. We then prospectively examined the expression of vasopressin receptor genes in adrenocortical tumors of recently operated patients (20 adenomas and 19 adrenocortical carcinomas). We used highly sensitive and specific quantitative RT-PCR techniques for each of the newly characterized human vasopressin receptors: V1, V2, and V3. The V1 messenger ribonucleic acid (mRNA) was detected in normal adrenal cortex and in all tumors. Its level varied widely between 2.0 x 10(2) and 4.4 x 10(5) copies/0.1 microgram total RNA, and adenomas had significantly higher levels than carcinomas, although there was a large overlap. Among the 6 recently operated patients who had been subjected to the vasopressin test in vivo, the tumor V1 mRNA levels were higher in the 4 responders (9.5 x 10(3) to 5.0 x 10(4)) than in the 2 nonresponders (2.0 x 10(2) and 1.8 x 10(3)). One adenoma that had a brisk cortisol response in vivo, also had in vitro cortisol responses that were inhibited by a specific V1 antagonist. In situ hybridization showed the presence of V1 mRNA in the normal human adrenal cortex where the signal predominated in the compact cells of the zona reticularis. A positive signal was also present in the tumors with high RT-PCR V1 mRNA levels; its distribution pattern was heterogeneous and showed preferential association with compact cells. RT-PCR studies for the other vasopressin receptors showed a much lower signal for V2 and no evidence for V3 mRNA. We could not establish whether the V2 mRNA signal observed in normal and tumoral specimens was present within adrenocortical cells or merely within tissue vessels. We conclude that the vasopressin V1 receptor gene is expressed in normal and tumoral adrenocortical cells. High, and not ectopic, expression occurs in a minority of tumors that become directly responsive to vasopressin stimulation tests.

Topics: Adenoma; Adrenal Cortex Hormones; Adrenal Cortex Neoplasms; Carcinoma; Cushing Syndrome; Gene Expression; Humans; Hydrocortisone; In Situ Hybridization; Lypressin; Phenotype; Polymerase Chain Reaction; Receptors, Vasopressin; Retrospective Studies; RNA-Directed DNA Polymerase; RNA, Messenger; Vasopressins

We achieved histological detection of the messenger RNAs coding for vasopressin, calcitonin, or calcitonin gene-related peptide by using biotinylated synthetic oligonucleotides, and defined the technical parameters enabling optimal detection of these mRNAs. Oligonucleotides labeled by fixation of one biotin at their 5' end or by addition of a biotin-11-dUTP tail at their 3' end can be used to detect mRNAs, although the latter are more sensitive. Streptavidin-alkaline phosphatase revealed with nitroblue tetrazolium-bromo-chloro-indolyl phosphate as substrate makes possible detection of the biotinylated oligonucleotides. Increasing formaldehyde concentration in the fixative decreases the signal intensity; 1% formaldehyde fixation provides the most intense signal. Several controls, including those with addition of unlabeled oligonucleotides to the hybridization buffer, confirm the specificity of mRNA detection. The sensitivity of the biotinylated probes is identical or lower as compared to the corresponding radiolabeled oligonucleotides. Histological and subcellular resolution is greatly enhanced with biotinylated probes. The rat vasopressin probes stain magnocellular neurons in the supraoptic and paraventricular nuclei and, under optimal conditions, parvocellular neurons in the suprachiasmatic nucleus. Vasopressin mRNA is present in the cytoplasm of the cell bodies and in the roots of certain processes. Calcitonin and calcitonin gene-related peptide mRNA are found co-localized in the cytoplasm of the same tumor cells in human medullary thyroid carcinoma.

Topics: Animals; Biotin; Calcitonin; Calcitonin Gene-Related Peptide; Carcinoma; Fixatives; Hypothalamus; Male; Neuropeptides; Nucleic Acid Hybridization; Oligonucleotides; Rats; RNA, Messenger; Supraoptic Nucleus; Thyroid Neoplasms; Vasopressins

Forty-one patients with newly diagnosed small cell anaplastic lung cancer were evaluated for abnormalities in water homeostasis. Each patient underwent a standard water load (SWL) test. Overall, 68% had abnormalities in the SWL test. Abnormalities were found in 47% of the patients with carcinoma clinically limited to one hemithorax and in 86% of the patients with more extensive carcinoma. The determination of urinary antidiuretic hormone levels was available for 27 patients. Abnormally elevated levels were found in 44% of those patients. Forty-six patients had clinically detectable syndrome of the inappropriate secretion of antidiuretic hormone (SIADH); in 12% of patients water restriction was necessary. The incidence of detectable abnormalities in water homeostasis in this study was higher than has been previously recognized. The SWL test is a sensitive and useful means of determining the presence of impaired water handling in patients with small cell carcinoma of the lung.

Topics: Blood Urea Nitrogen; Carcinoma; Carcinoma, Bronchogenic; Drug Therapy, Combination; Female; Homeostasis; Humans; Inappropriate ADH Syndrome; Lung Neoplasms; Male; Methods; Vasopressins; Water; Water-Electrolyte Balance

A double paraneoplasic syndrome with hypersecretion of ADH and ACTH revealed the presence of a small cell bronchial cancer in a man aged 62 years. Water and electrolyte anomalies due to the hypersecretions were of such a degree that an occlusive syndrome occurred. Very high levels of ADH and ACTH were found in the tumoral tissue. The measurement of these levels is rarely conducted at the present time but was able to define the mechanism of abnormal secretion of these two hormones in this case.

Topics: Adrenocortical Hyperfunction; Adrenocorticotropic Hormone; APUD Cells; Carcinoma; Humans; Inappropriate ADH Syndrome; Lung Neoplasms; Male; Middle Aged; Paraneoplastic Syndromes; Vasopressins; Water-Electrolyte Imbalance

Topics: Adult; Bronchial Neoplasms; Carcinoma; Guanidines; Humans; Hyponatremia; Male; Muscular Diseases; Osmolar Concentration; Syndrome; Vasopressins

3 cases of inappropriate vasopressin secretion during one case of anaplastic carcinoma of the lung, one case of carcinoma of the prostate with bony metastases and one case of acute intermittent porphyria are presented. The plasma levels of vasopressin, measured by radioimmunoassay were high. Treatment with demeclocycline was attempted in one case. The clearance of free water was positive but the treatment was poorly tolerated by the digestive tract.

Topics: Acute Disease; Aged; Carcinoma; Demeclocycline; Female; Humans; Lung Neoplasms; Male; Paraneoplastic Endocrine Syndromes; Porphyrias; Prostatic Neoplasms; Syndrome; Vasopressins; Water-Electrolyte Imbalance

Topics: Adrenocorticotropic Hormone; Carcinoma; Chronic Disease; Female; Hormones, Ectopic; Humans; Lung Neoplasms; Middle Aged; Thyroiditis; Vasopressins

Topics: Adrenal Gland Neoplasms; Autopsy; Bone Neoplasms; Bronchial Neoplasms; Calcitonin; Carcinoma; Carcinoma, Small Cell; Humans; Hyponatremia; Liver Neoplasms; Lymphatic Metastasis; Male; Middle Aged; Neoplasm Metastasis; Osmolar Concentration; Paraneoplastic Endocrine Syndromes; Syndrome; Vasopressins

Topics: Adult; Bronchial Neoplasms; Carcinoma; Diagnosis, Differential; Humans; Hyponatremia; Lung Neoplasms; Male; Muscular Diseases; Muscular Dystrophies; Osmolar Concentration; Syndrome; Vasopressins

Topics: Adenocarcinoma; Adrenocortical Hyperfunction; Adrenocorticotropic Hormone; Bronchial Neoplasms; Carcinoma; Female; Gastrointestinal Neoplasms; Gonadotropins; Growth Hormone; Hormones, Ectopic; Humans; Hyperparathyroidism; Hyperthyroidism; Lung Neoplasms; Male; Paraneoplastic Endocrine Syndromes; Parathyroid Hormone; Pituitary Hormones; Sex Factors; Thymus Neoplasms; Thyroid Neoplasms; Thyrotropin; Urogenital Neoplasms; Vasopressins

Topics: Aged; Carcinoma; Humans; Lung Neoplasms; Male; Microscopy, Electron; Paraneoplastic Endocrine Syndromes; Vasopressins

Topics: Adrenal Gland Neoplasms; Carcinoma; Female; Humans; Hyponatremia; Middle Aged; Neoplasm Metastasis; Osmolar Concentration; Sodium; Syndrome; Vasopressins

Topics: Adrenocorticotropic Hormone; Biological Assay; Calcitonin; Carcinoma; Depression, Chemical; Dexamethasone; Fluorescent Antibody Technique; Humans; Hydrocortisone; Male; Middle Aged; Neoplasm Metastasis; Parathyroid Hormone; Radioimmunoassay; Secretory Rate; Stimulation, Chemical; Thyroid Neoplasms; Vasopressins

Topics: Adenosine Triphosphate; Adenylyl Cyclases; Adrenal Gland Neoplasms; Adrenal Glands; Adrenocorticotropic Hormone; Amines; Amino Acid Sequence; Animals; Benzyl Compounds; Binding Sites; Calcitonin; Carcinoma; Catalysis; Catecholamines; Depression, Chemical; Epinephrine; Female; Follicle Stimulating Hormone; Glucagon; Hormones; Humans; Luteinizing Hormone; Male; Metabolism; Methoxamine; Neoplasms, Experimental; Norepinephrine; Parathyroid Hormone; Phentolamine; Phosphorus Isotopes; Propranolol; Rats; Receptors, Adrenergic; Stimulation, Chemical; Thyrotropin; Vasopressins

Topics: Bronchial Neoplasms; Carcinoma; Carcinoma, Bronchogenic; Hormones, Ectopic; Humans; Hyponatremia; Hypoventilation; Lung Neoplasms; Male; Middle Aged; Muscular Diseases; Myositis; Vasopressins

Topics: Carcinoma; Diagnosis, Differential; Exudates and Transudates; Female; Humans; Hyponatremia; Intestinal Obstruction; Middle Aged; Myxedema; Osmolar Concentration; Pelvic Inflammatory Disease; Pelvic Neoplasms; Sodium; Thyroid Hormones; Vasopressins; Water Intoxication; Water-Electrolyte Balance

Topics: Breast Neoplasms; Carcinoma; Deficiency Diseases; Diabetes Insipidus; Female; Humans; Injections, Subcutaneous; Male; Neoplasm Metastasis; Osmolar Concentration; Pituitary Function Tests; Pituitary Gland, Posterior; Pituitary Neoplasms; Polyuria; Vasopressins; Water Deprivation

Topics: Bronchial Neoplasms; Carcinoma; Electroencephalography; Humans; Hyponatremia; Middle Aged; Natriuresis; Neoplasm Metastasis; Vasopressins; Water Intoxication

Topics: Adrenocorticotropic Hormone; Animals; Biological Assay; Calcitonin; Carcinoma; Chickens; Humans; Immune Sera; Iodine Isotopes; Methods; Oxytocin; Parathyroid Hormone; Radioimmunoassay; Swine; Thyroid Gland; Thyroid Neoplasms; Tissue Extracts; Vasopressins

Topics: Adult; Biopsy; Carcinoma; Female; Hemostatics; Humans; Infant, Newborn; Methods; Pregnancy; Pregnancy Complications; Uterine Cervical Diseases; Uterine Cervical Neoplasms; Vasopressins

Topics: Adrenocortical Hyperfunction; Adrenocorticotropic Hormone; Aldosterone; Alkalosis; Bronchial Neoplasms; Carcinoma; Endocrine System Diseases; Fludrocortisone; Humans; Hypercalcemia; Hypokalemia; Hyponatremia; Male; Plasma Volume; Vasopressins

Topics: Blood Pressure; Carcinoma; Chromatography, Gel; Countercurrent Distribution; Humans; Lung Neoplasms; Male; Middle Aged; Vasopressins

Topics: Aged; Animals; Anura; Bronchial Neoplasms; Carcinoma; Humans; Hyponatremia; Hypopituitarism; Male; Neoplasm Metastasis; Pancreatic Neoplasms; Permeability; Sodium; Urinary Bladder; Vasopressins; Water

Topics: Aldosterone; Blood Volume; Bronchial Neoplasms; Carcinoma; Cyclophosphamide; Diuresis; Electroencephalography; Hormones, Ectopic; Humans; Hyponatremia; Male; Middle Aged; Natriuresis; Plasma Volume; Radioisotope Teletherapy; Renin; Vasopressins

Topics: Aldosterone; Carcinoma; Glomerular Filtration Rate; Humans; Hyponatremia; Lung Neoplasms; Male; Middle Aged; Vasopressins; Water-Electrolyte Balance

Topics: Arginine; Carcinoma; Female; Humans; Hyponatremia; Immunoassay; In Vitro Techniques; Lung Neoplasms; Middle Aged; Natriuresis; Tissue Extracts; Vasopressins

Topics: Adult; Aldosterone; Bronchial Neoplasms; Carcinoma; Humans; Hyponatremia; Male; Vasopressins

Topics: Carcinoma; Carcinoma, Bronchogenic; Fluids and Secretions; Humans; Metabolism; Urine; Vasopressins

Topics: Arginine Vasopressin; Bronchial Neoplasms; Carcinoma; Carcinoma, Bronchogenic; Drug Therapy; Fluids and Secretions; Humans; Hyponatremia; Mechlorethamine; Neoplasms; Urine; Vasopressins

Topics: Blood Chemical Analysis; Bronchial Neoplasms; Carcinoma; Humans; Hyponatremia; Metabolism; Neoplasms; Urine; Vasopressins

Topics: Biological Assay; Blood; Carcinoma; Carcinoma, Bronchogenic; Humans; Liver Neoplasms; Neoplasm Metastasis; Vasopressins

Topics: Arginine Vasopressin; Carcinoma; Humans; Hyponatremia; Inappropriate ADH Syndrome; Lung Neoplasms; Metabolism; Vasopressins; Water-Electrolyte Balance

Topics: Arginine Vasopressin; Blood Chemical Analysis; Carcinoma; Carcinoma, Small Cell; Geriatrics; Humans; Hyponatremia; Lung Neoplasms; Vasopressins

Topics: Autonomic Nerve Block; Carcinoma; Chlorpromazine; Geriatrics; Histocytochemistry; Humans; Kidney Diseases; Lung Neoplasms; Neoplasm Metastasis; Neoplasms; Nitrogen Mustard Compounds; Vasopressins

Topics: Carcinoma; Carcinoma, Bronchogenic; Humans; Hyponatremia; Lung Neoplasms; Mediastinal Neoplasms; Renal Elimination; Urine; Vasopressins; Water-Electrolyte Balance

Topics: Carcinoma; Carcinoma, Bronchogenic; Humans; Hyponatremia; Sodium; Vasopressins

Topics: Carcinoma; Carcinoma, Bronchogenic; Diabetes Insipidus, Neurogenic; Humans; Hyponatremia; Kidney; Sodium; Vasopressins