piperine and Ovarian-Neoplasms

Our goal was to examine the anticancer effects of piperine against the resistant human ovarian cancer cells and to explore the molecular mechanisms responsible for its anticancer effects. Our study used drug-sensitive ovarian cancer cell line W1 and its sublines resistant to paclitaxel (PAC) and topotecan (TOP). We analyzed the cytotoxic effect of piperine and cytostatic drugs using an MTT assay. The impact of piperine on protein expression was determined by immunofluorescence and Western blot. We also examined its effect on cell proliferation and migration. We noticed a different level of piperine resistance between cell lines. Piperine increases the cytotoxic effect of PAC and TOP in drug-resistant cells. We observed an increase in PTPRK expression correlated with decreased pTYR level after piperine treatment and downregulation of P-gp and BCRP expression. We also noted a decrease in COL3A1 and TGFBI expression in investigated cell lines and increased COL3A1 expression in media from W1PR2 cells. The expression of Ki67 protein and cell proliferation rate decreased after piperine treatment. Piperine markedly inhibited W1TR cell migration. Piperine can be considered a potential anticancer agent that can increase chemotherapy effectiveness in cancer patients.

Topics: Aldehyde Dehydrogenase 1 Family; Alkaloids; Antineoplastic Agents; Benzodioxoles; Cell Line, Tumor; Cell Survival; Collagen Type III; Drug Resistance, Neoplasm; Extracellular Matrix Proteins; Female; Humans; Ovarian Neoplasms; Paclitaxel; Phosphorylation; Piperidines; Polyunsaturated Alkamides; Retinal Dehydrogenase; Transforming Growth Factor beta

Topics: Alkaloids; Anthracenes; Apoptosis; Benzodioxoles; Caspase 3; Caspase 9; Caspase Inhibitors; Cell Proliferation; Cell Survival; Female; Humans; Imidazoles; MAP Kinase Kinase 4; Oligopeptides; Ovarian Neoplasms; p38 Mitogen-Activated Protein Kinases; Piperidines; Polyunsaturated Alkamides; Pyridines; Tumor Suppressor Proteins