piperine and Mastitis

Mastitis, inflammation in the breast, affects breastfeedingwomenin the postpartumperiod. In the present study, we investigated the protective effects of piperine against mastitis using a mouse mastitis model. LPS-induced mastitis was established by injecting LPS into the canals of the mammary gland. Piperine was given intraperitoneally 1 h before and 12 h after LPS treatment. The results showed that the LPS-induced mammary histopathological changes and MPO activity were attenuated by piperine. LPS-induced inflammatory cytokines TNF-α andIL-1β were also inhibited by piperine. Furthermore, LPS-induced NF-κB activation was suppressed by the treatment with piperine. In addition, we found piperine dose-dependently increased the expression of PPARγ. All of these results suggested that piperine had protective effects against LPS-induced mastitis and that the mechanism may be mediated through the activation of PPARγ.

Topics: Alkaloids; Animals; Anti-Inflammatory Agents; Benzodioxoles; Disease Models, Animal; Female; Humans; Immunohistochemistry; Interleukin-1beta; Lipopolysaccharides; Mammary Glands, Human; Mastitis; Mice; Mice, Inbred BALB C; NF-kappa B; Peroxidase; Piperidines; Polyunsaturated Alkamides; PPAR gamma; Tumor Necrosis Factor-alpha; Up-Regulation