piperidines and Sarcoma-180

It has been reported that piplartine and piperine, alkaloid/amide compounds from Piper species, show antitumor activities. In the present paper, the effects of the combination of 5-fluorouracil (5-FU) with piplartine or piperine was determined using in vitro and in vivo experimental models. Hematological and biochemical analyses, as well as histopathological and morphological analyses of the tumor and the organs, including liver, spleen and kidney, were performed in order to evaluate the toxicological aspects associated with different treatments. The incubation of tumor cell lines with 5-FU in the presence of piplartine or piperine produced an increase in growth inhibition, as observed by lower IC50 values for 5-FU. These effects were also observed in vivo, where the combination with piplartine but not piperine with 5-FU led to a higher tumor growth inhibition. The results indicated that either piplartine- or 5-FU-treated animals showed a low inhibition rate when they were used individually at low doses of 28.67% and 47.71%, respectively, but when they were combined at the same dose, the inhibition rate increased significantly to 68.04%. The histopathological analysis showed that the livers and the kidneys of treated animals were only slightly and reversibly affected. Neither the enzymatic activity of transaminases nor the urea levels were significantly modified when compared with the control group. Hematological analysis showed leukopenia after 5-FU treatment, which was reversed by the combined use of piplartine and piperine. These findings indicate that piplartine may enhance the therapeutic effectiveness of chemotherapeutic drugs, and moreover, this combination could improve immunocompetence hampered by 5-FU.

Topics: Alanine Transaminase; Alkaloids; Animals; Antimetabolites, Antineoplastic; Antineoplastic Agents, Phytogenic; Antineoplastic Combined Chemotherapy Protocols; Aspartate Aminotransferases; Benzodioxoles; Cell Proliferation; Dose-Response Relationship, Drug; Female; Fluorouracil; HL-60 Cells; Humans; Inhibitory Concentration 50; Kidney; Leukopenia; Liver; Mice; Piperidines; Piperidones; Polyunsaturated Alkamides; Sarcoma 180; Urea

Piplartine {5,6-dihydro-1-[1-oxo-3-(3,4,5-trimethoxyphenyl)-2-propenyl]-2(1H)pyridinone} and piperine {1-5-(1,3)-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine} are alkaloid amides isolated from Piper. Both have been reported to show cytotoxic activity towards several tumor cell lines. In the present study, the in vivo antitumor activity of these compounds was evaluated in 60 female Swiss mice (N = 10 per group) transplanted with Sarcoma 180. Histopathological and morphological analyses of the tumor and the organs, including liver, spleen, and kidney, were performed in order to evaluate the toxicological aspects of the treatment with these amides. Administration of piplartine or piperine (50 or 100 mg kg(-1) day(-1) intraperitoneally for 7 days starting 1 day after inoculation) inhibited solid tumor development in mice transplanted with Sarcoma 180 cells. The inhibition rates were 28.7 and 52.3% for piplartine and 55.1 and 56.8% for piperine, after 7 days of treatment, at the lower and higher doses, respectively. The antitumor activity of piplartine was related to inhibition of the tumor proliferation rate, as observed by reduction of Ki67 staining, a nuclear antigen associated with G1, S, G2, and M cell cycle phases, in tumors from treated animals. However, piperine did not inhibit cell proliferation as observed in Ki67 immunohistochemical analysis. Histopathological analysis of liver and kidney showed that both organs were reversibly affected by piplartine and piperine treatment, but in a different way. Piperine was more toxic to the liver, leading to ballooning degeneration of hepatocytes, accompanied by microvesicular steatosis in some areas, than piplartine which, in turn, was more toxic to the kidney, leading to discrete hydropic changes of the proximal tubular and glomerular epithelium and tubular hemorrhage in treated animals.

Topics: Alkaloids; Animals; Antineoplastic Agents, Phytogenic; Benzodioxoles; Cell Proliferation; Disease Models, Animal; Female; Kidney; Liver; Mice; Neoplasm Transplantation; Piper; Piperidines; Piperidones; Plant Extracts; Plant Roots; Polyunsaturated Alkamides; Sarcoma 180; Spleen

Topics: Animals; Antineoplastic Agents; Carcinoma, Ehrlich Tumor; Carcinoma, Hepatocellular; Drug Evaluation, Preclinical; Female; In Vitro Techniques; Leukemia L1210; Liver Neoplasms; Mice; Neoplasms, Experimental; Piperidines; Rats; Sarcoma 180; Sarcoma, Yoshida

Topics: Animals; Anti-Bacterial Agents; Bacteria; Candida; Infrared Rays; Magnetic Resonance Spectroscopy; Mice; Mycobacterium; Piperidines; Saccharomyces; Sarcoma 180; Ultraviolet Rays

Topics: Animals; Anti-Bacterial Agents; Antineoplastic Agents; Chemistry, Pharmaceutical; Ketones; Leukemia L1210; Neoplasms, Experimental; Pharmacology; Piperidines; Piperidones; Research; Sarcoma 180

Topics: Animals; Antineoplastic Agents; Blood; Carcinoma, Ehrlich Tumor; DNA; DNA, Neoplasm; Mice; Neoplasms; Neoplasms, Experimental; Piperidines; Prochlorperazine; Rabbits; Research; Reserpine; RNA; RNA, Neoplasm; Sarcoma 180

Topics: 2-Aminopurine; Animals; Antineoplastic Agents; Chemistry, Pharmaceutical; Leukemia; Leukemia, Experimental; Morpholines; Pharmacology; Piperidines; Purines; Pyrimidines; Pyrroles; Research; Sarcoma 180