picogreen and Huntington-Disease

picogreen has been researched along with Huntington-Disease* in 1 studies

Other Studies

1 other study(ies) available for picogreen and Huntington-Disease

Article	Year
Microtiter plate quantification of mutant and wild-type huntingtin normalized to cell count. Analytical biochemistry, 2011, Mar-15, Volume: 410, Issue:2 Huntington's disease is caused by a gain-of-function neurotoxic mutation in normally neuroprotective huntingtin. Sensitive assays are required to discriminate mutant huntingtin from wild-type huntingtin. We have developed a normalized 384-plate assay for determination of mutant and wild-type huntingtin. Based on a single pipetting step, the sensitive assay uses two antibody pairs for simultaneous mutant and wild-type huntingtin time-resolved fluorescence resonance energy transfer detection combined with PicoGreen quantification of double-stranded DNA. The assay can be used for discovery of drugs reducing mutant huntingtin over wild-type huntingtin and for assessing the value of huntingtin as a disease progression marker, and it is adaptable to other proteins of interest. Topics: Antibodies; Cell Count; Cell Line; DNA; Fibroblasts; Fluorescence Resonance Energy Transfer; Humans; Huntingtin Protein; Huntington Disease; Mutant Proteins; Nerve Tissue Proteins; Nuclear Proteins; Organic Chemicals	2011

Article

Year

Microtiter plate quantification of mutant and wild-type huntingtin normalized to cell count.

Analytical biochemistry, 2011, Mar-15, Volume: 410, Issue:2

Huntington's disease is caused by a gain-of-function neurotoxic mutation in normally neuroprotective huntingtin. Sensitive assays are required to discriminate mutant huntingtin from wild-type huntingtin. We have developed a normalized 384-plate assay for determination of mutant and wild-type huntingtin. Based on a single pipetting step, the sensitive assay uses two antibody pairs for simultaneous mutant and wild-type huntingtin time-resolved fluorescence resonance energy transfer detection combined with PicoGreen quantification of double-stranded DNA. The assay can be used for discovery of drugs reducing mutant huntingtin over wild-type huntingtin and for assessing the value of huntingtin as a disease progression marker, and it is adaptable to other proteins of interest.

Topics: Antibodies; Cell Count; Cell Line; DNA; Fibroblasts; Fluorescence Resonance Energy Transfer; Humans; Huntingtin Protein; Huntington Disease; Mutant Proteins; Nerve Tissue Proteins; Nuclear Proteins; Organic Chemicals

2011