picibanil and Prostatic-Neoplasms

Dendritic cells (DCs) are potent antigen-presenting cells. OK432 (Picibanil) was introduced as a potent stimulator of DC maturation in combination with prostaglandin-E(2) and interferon-alpha. We compared the efficacy of a DC-prostate cancer vaccine using early-mature DCs stimulated with OK432, PGE2 and INF-alpha (OPA) with that of vaccines using other methods. On days 3 or 7 of DC culture, TNF-alpha (T), TNF-alpha and LPS (TL) or OPA were employed as maturation stimulators. DU145 cells subjected to heat stress were hybridized with mature DCs using polyethyleneglycol. T cells were sensitized by the hybrids, and their proliferative and cytokine secretion activities and cytotoxicity were measured. The yields of early-mature DCs were higher, compared to yields at the conventional maturation time (P<0.05). In the early maturation setting, the mean fusion ratios, calculated from the fraction of dual-positive cells, were 13.3%, 18.6%, and 39.9%, respectively (P=0.051) in the T only, TL, and OPA-treated groups. The function of cytotoxic T cells, which were sensitized with the hybrids containing DCs matured early with OPA, was superior to that using other methods. The antitumor effects of DC-DU145 hybrids generated with DCs subjected to early maturation with the OPA may be superior to that of the hybrids using conventional maturation methods.

Topics: Cancer Vaccines; Cell Line, Tumor; Dendritic Cells; Dinoprostone; Humans; Immunologic Factors; Interferon-alpha; Lipopolysaccharides; Male; Neoplasms, Hormone-Dependent; Phenotype; Picibanil; Prostatic Neoplasms; T-Lymphocytes, Cytotoxic

Topics: Biological Products; Cell Line; Cytotoxicity, Immunologic; Humans; Immunotherapy; Interferon Inducers; Kidney Neoplasms; Killer Cells, Natural; Male; Picibanil; Prostatic Neoplasms

Topics: Adenocarcinoma; Aged; Drug Therapy, Combination; Hexestrol; Humans; Male; Middle Aged; Mitomycins; Picibanil; Prostatic Neoplasms