picibanil and Infertility--Female

To investigate the utility of using oily contrast medium (Lipiodol; Kodama, Tokyo, Japan) to treat infertility because of mild endometriosis.. Macrophages in the peritoneal cavity of ICR mice (Japan SLC, Shizuoka, Japan) were induced by administering OK-432 to simulate the condition of the peritoneal cavity of patients with mild endometriosis, and the pregnancy rate was examined after administration of Lipiodol and/or danazol (Bonzol; Tokyo Tanabe, Tokyo, Japan). Interleukin-1 beta (IL-1 beta), prostaglandin (PG)F2 alpha (PGF2 alpha), and PGE2 levels in the supernatant were measured after incubating macrophages of mice with Lipiodol and/or danazol.. Pregnancy rate (PR) and IL-1 beta, PGF2 alpha, and PGE2 levels.. The PR of the Lipiodol group was significantly increased compared with that in control mice. Prostaglandin F2 alpha level in the supernatant was significantly increased, whereas that of IL-1 beta was reduced after incubation with Lipiodol.. Lipiodol may elevate PGF2 alpha level and reduce IL-1 beta level in the peritoneal cavity, and this drug may increase the PR of ICR mice.

Topics: Animals; Cells, Cultured; Danazol; Dinoprost; Dinoprostone; Endometriosis; Female; Infertility, Female; Interleukin-1; Iodized Oil; Macrophages; Male; Mice; Mice, Inbred ICR; Peritoneal Cavity; Picibanil; Pregnancy

In investigating the cause of infertility in patients with endometriosis, not only organic disturbances, but also other factors must be considered. I here discuss the participation of interleukin-1 (IL-1) in peritoneal fluid (PF) in infertility and embryonal developmental failure in animal experiment. I next investigated the mechanism for this in mice. The number of corpora lutea and the state of cleavage of the fertilized ovum 96 hours after hCG administration under influence of M phi and IL-1 were investigated. Further in a culture system of human endometrial interstitial cells, the content of intracellular glycogen, which is a source of energy for the growth of the fertilized ovum after implantation, as well as the influence of IL-1 on enzymes metabolizing intracellular glycogen were investigated. As the result, it is considered that M phi in the peritoneal cavity secrete IL-1, which has no influence on ovulation, but inhibits cleavage of the fertilized ovum, decreases the content of intracellular glycogen in the endometrium, and disturbs the environment for ovular growth after implantation, resulting in infertility.

Topics: Animals; Endometriosis; Female; Infertility, Female; Interleukin-1; Macrophages; Mice; Mice, Inbred ICR; Peritoneal Cavity; Picibanil