picibanil and Hepatitis--Chronic

Cytokines production by OK-432-stimulated peripheral blood mononuclear cells (PBMC) were measured to investigate the in vitro function of macrophages (M phi) and lymphocytes. PBMC (1 x 10(6) cells/ml) were cultured with OK-432 (0.05 KE/ml) for 72 hr at 37 degrees C under 5% CO2, then interleukin 1 beta (IL-1 beta), interleukin 2 (IL-2) and soluble IL-2 receptor (sIL-2R) levels in the culture supernatants were measured by ELISA. While there was no significant differences of IL-1 beta production between patients with chronic active hepatitis type B (CAH-B) and controls, sIL-2R production (335 +/- 219 U/ml, mean +/- SD) was significantly decreased (p < 0.001) in patients with CAH-B. On the other hand, in pregnant women, production of both IL-1 beta (6.3 +/- 3.9 ng/ml, p < 0.01) and sIL-2R (300 +/- 169 U/ml, p < 0.001) were significantly lower than those in controls (13.5 +/- 3.8 ng/ml, 969 +/- 154 U/ml). These results suggest that the expression of IL-2R alpha on lymphocytes membrane is suppressed in patients with CAH-B, and that decreased M phi function is present in pregnant women.

Topics: Cells, Cultured; Cytokines; Female; Hepatitis B; Hepatitis, Chronic; Humans; Leukocytes, Mononuclear; Picibanil; Pregnancy

Twenty-two patients with chronic type B hepatitis were treated with OK-432. Immunological parameters were serially measured to find predictive indicators for the seroconversion from hepatitis B envelope antigen (HBe Ag) to anti-HBe. In patients who achieved the disappearance of HBe Ag associated with or without the appearance of anti-HBe, the numbers of CD8+DR+ and CD4+DR+T cells in peripheral blood increased gradually during OK-432 therapy and then reduced subsequently to the seroconversion from HBe Ag positive to anti-HBe positive. Increases of DR-positive T cells in numbers were significantly correlated with increased amounts of IFN-gamma produced in response to in vitro OK-432 stimulation. In vitro OK-432-stimulated IFN-gamma production and the increase of CD8+DR+T cells in number in peripheral blood could be proposed as predictive indicators for the disappearance of HBe Ag.

Topics: Adolescent; Adult; Alanine Transaminase; Female; Flow Cytometry; Hepatitis B; Hepatitis B e Antigens; Hepatitis, Chronic; HLA-DR Antigens; Humans; Interferon-gamma; Male; Middle Aged; Picibanil; Predictive Value of Tests; Prognosis; T-Lymphocyte Subsets; T-Lymphocytes

Topics: Adjuvants, Immunologic; Adult; Alanine Transaminase; Biological Products; Drug Evaluation; Female; Hepatitis C; Hepatitis, Chronic; Hepatitis, Viral, Human; Humans; Liver; Male; Middle Aged; Picibanil