phytosterols and Psoriasis

Topics: Animals; Inflammation; Mice; Molecular Docking Simulation; Phytosterols; Psoriasis; Sitosterols; Stigmasterol; Swine

Khasianine is recently identified as a bioactive compound from Solanum nigrum L. (SNL) which is a traditional Chinese herb (named LongKui in China) and has been clinically applied for treating psoriasis in China but with limited knowledge about the active ingredients.. This study tried to explore the bioactivity of Khasianine and showed that Khasianine possessed highly anti-inflammatory bioactivity which rapidly alleviated psoriasis-like mice skin inflammation.. Imiquimod induced psoriasis-like mouse model, and human keratinocytes were employed in this study. In vivo, immunohistochemistry and immunofluorescence were performed to evaluate the pathological improvement in psoriatic lesions after Khasianine treatment. In vitro, tumor necrosis factor α (TNF-α) treated HaCaT cells with or without Khasianine, were used to analyze the expression and cellular location of NF-κB p65, the expression of IL-17A and IL-33, and the binding intensity of NF-κB p65 on the promoter of IL-17A and IL-33 to understand the molecular mechanism of Khasianine mediated anti-inflammatory effect.. Khasianine reduced infiltration of CD4. Our results suggested that Khasianine is a potent anti-inflammatory compound with the bioactivity of NF-κB inhibition and is a promising candidate for psoriasis topical therapy.

Topics: Animals; Anti-Inflammatory Agents; Dermatitis; Interleukin-17; Interleukin-33; Keratinocytes; Mice; Mice, Inbred BALB C; NF-kappa B; Phytosterols; Psoriasis; Skin; Solanaceous Alkaloids; Transcriptional Activation; Tumor Necrosis Factor-alpha