phytochlorin and Uterine-Cervical-Neoplasms

Multi-model combination treatment of malignant tumors can make up for the shortcomings of single treatment through multi-target and multi-path to achieve more ideal tumor treatment effect. However, the mutual interference of different drugs in the delivery process

Topics: Cell Line, Tumor; Doxorubicin; Female; Humans; Liposomes; Nanoparticles; Photochemotherapy; Photosensitizing Agents; Uterine Cervical Neoplasms

The aim of this study is to prepare redox-sensitive nanophotosensitizers for the targeted delivery of chlorin e6 (Ce6) against cervical cancer. For this purpose, Ce6 was conjugated with β-cyclodextrin (bCD) via a disulfide bond, creating nanophotosensitizers that were fabricated for the redox-sensitive delivery of Ce6 against cancer cells. bCD was treated with succinic anhydride to synthesize succinylated bCD (bCDsu). After that, cystamine was attached to the carboxylic end of bCDsu (bCDsu-ss), and the amine end group of bCDsu-ss was conjugated with Ce6 (bCDsu-ss-Ce6). The chemical composition of bCDsu-ss-Ce6 was confirmed with

Topics: Animals; beta-Cyclodextrins; Cell Line, Tumor; Chlorophyllides; Female; HeLa Cells; Humans; Nanoparticles; Oxidation-Reduction; Photochemotherapy; Photosensitizing Agents; Porphyrins; Reactive Oxygen Species; Uterine Cervical Neoplasms

The aim of this study was to fabricate a reactive oxygen species (ROS)-sensitive and folate-receptor-targeted nanophotosensitizer for the efficient photodynamic therapy (PDT) of cervical carcinoma cells. Chlorin e6 (Ce6) as a model photosensitizer was conjugated with succinyl β-cyclodextrin via selenocystamine linkages. Folic acid (FA)-poly(ethylene glycol) (PEG) (FA-PEG) conjugates were attached to these conjugates and then FA-PEG-succinyl β-cyclodextrin-selenocystamine-Ce6 (FAPEGbCDseseCe6) conjugates were synthesized. Nanophotosensitizers of FaPEGbCDseseCe6 conjugates were fabricated using dialysis membrane. Nanophotosensitizers showed spherical shapes with small particle sizes. They were disintegrated in the presence of hydrogen peroxide (H

Topics: Animals; beta-Cyclodextrins; Cell Line; Cell Line, Tumor; Chlorophyllides; Female; Folate Receptors, GPI-Anchored; Folic Acid; HeLa Cells; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Nanoparticles; Oxidation-Reduction; Particle Size; Photochemotherapy; Photosensitizing Agents; Porphyrins; Uterine Cervical Neoplasms

Topics: Animals; Cell Proliferation; Cell Survival; Chlorophyllides; Endocytosis; Female; Ferrocyanides; HeLa Cells; Humans; Hyaluronan Receptors; Hyaluronic Acid; Indoles; Magnetic Resonance Imaging; Mice; Nanoparticles; NIH 3T3 Cells; Photochemotherapy; Photosensitizing Agents; Polymers; Porphyrins; Theranostic Nanomedicine; Uterine Cervical Neoplasms; Xenograft Model Antitumor Assays

Topics: Animals; Blood Circulation; Cell Survival; Chlorophyllides; Doxorubicin; Drug Liberation; Female; Glutathione; HeLa Cells; Humans; Hydrogen-Ion Concentration; Manganese Compounds; Mice, Nude; Nanoparticles; Oxides; Porosity; Porphyrins; Rats, Sprague-Dawley; Serum Albumin, Bovine; Silicon Dioxide; Singlet Oxygen; Tumor Hypoxia; Uterine Cervical Neoplasms

Spherical nucleic acid (SNA) constructs are promising new single entity materials, which possess significant advantages in biological applications. Current SNA-based drug delivery system typically employed single-layered ss- or ds-DNA as the drug carriers, resulting in limited drug payload capacity and disease treatment. To advance corresponding applications, we developed a novel DNA-programmed polymeric SNA, a long concatamer DNA polymer that is uniformly distributed on gold nanoparticles (AuNPs), by self-assembling from two short alternating DNA building blocks upon initiation of immobilized capture probes on AuNPs, through a supersandwich hybridization reaction. The long DNA concatamer of polymeric SNA enables to allow high-capacity loading of bioimaging and therapeutics agents. We demonstrated that both of the fluorescence signals and therapeutic efficacy were effectively inhibited in resultant polymeric SNA. By further modifying with the nucleolin-targeting aptamer AS1411, this polymeric SNA could be specifically internalized into the tumor cells through nucleolin-mediated endocytosis and then interact with endogenous ATP to cause the release of therapeutics agents from long DNA concatamer via a structure switching, leading to the activation of the fluorescence and selective synergistic chemotherapy and photodynamic therapy. This nanostructure can afford a promising targeted drug transport platform for activatable cancer theranostics.

Topics: Adenosine Triphosphate; Antineoplastic Agents; Aptamers, Nucleotide; Chlorophyllides; DNA; Doxorubicin; Drug Carriers; Drug Liberation; Female; Fluorescence; Gold; HeLa Cells; Humans; Light; Metal Nanoparticles; Microscopy, Confocal; Nucleic Acid Hybridization; Oligodeoxyribonucleotides; Photosensitizing Agents; Porphyrins; Singlet Oxygen; Theranostic Nanomedicine; Uterine Cervical Neoplasms

Novel photoinduced triple-response antitumor therapeutic system based on hollow gold nanospheres (HAuNS), pH (low) insertion peptide (pHLIP), and Chlorin e6 (Ce6), was reported for the first time. The system was able to intracellularly deliver the nanocarriers by the transmembrane ability of pHLIP at the condition of pH 6.2. Ce6 and pHLIP were then released from the surface of the carriers due to the weakening electrostatic interaction with HAuNS under the photoirradiation. Herein, HAuNS performed two different functions: (1) as a nanocarrier because of the excellent loading capability; (2) experienced the photothermal therapy (PTT) effect as a photothermal coupling agent (PTCA), thus enhancing the photodynamic therapy (PDT) effect of Ce6.

Topics: Animals; Chlorophyllides; Drug Carriers; Female; Gold; HeLa Cells; Humans; Hydrogen-Ion Concentration; Light; Mice; Mice, Nude; Nanospheres; Peptides; Photochemotherapy; Photosensitizing Agents; Porphyrins; Temperature; Transplantation, Heterologous; Uterine Cervical Neoplasms

A supramolecular drug delivery system has been developed via the self-assembly of a supramolecular amphiphilic polymer, which is constructed by the host-guest interaction of hydrophilic PEGylated calix[4]arene and hydrophobic photosensitizer chlorin e6. It provides a new strategy for the preparation of supramolecular polymeric micelles, and plays an important role in biological applications.

Topics: Calixarenes; Chlorophyllides; Female; HeLa Cells; Humans; Micelles; Phenols; Photochemotherapy; Polyethylene Glycols; Porphyrins; Uterine Cervical Neoplasms

The objective of the present study was to test in clinics a previously developed novel organ-saving approach for the treatment of CIN using PDT with the photosensitizer Photolon applied in women of a childbearing age with CIN II and III. A total number of 112 patients aged 35.2+/-1.6 with morphologically proven diagnosis of CIN II and III were enrolled into the study. All 112 patients had been observed at least during 1-year follow-up period after PDT. Among them 53 patients (44.1%) were subjected to a dynamic observation for less than 2 years; 29 patients (24.1%) were under the observation for less than 3 years; 13 patients (10.8%) - for 3-4 years and 17 women - for more than 4 years. A complete response represented by the complete regression of neoplastic lesions, which was proved by the results of morphological examinations, was revealed in 104 (92.8%) of treated women. In 3 months after treatment a complete eradication of the HPV infection was proven by PCR-analysis in 47 (53.4%) from 88 patients who have been infected with HPV of a highly oncogenic strains before PDT. PDT with Photolon is an alternative approach for the treatment of cervical intraepithelial neoplasia which can be recommended for women of childbearing age. The simplicity of the procedure as well as its' high therapeutic efficacy defines the reasonability of its' introduction into the clinical practice as a new organ-saving method for the treatment of patients with cervical intraepithelial neoplasia.

Topics: Adult; Chlorophyllides; Female; Humans; Neoplasm Staging; Papillomavirus Infections; Photochemotherapy; Photosensitizing Agents; Porphyrins; Povidone; Protoporphyrins; Uterine Cervical Dysplasia; Uterine Cervical Neoplasms